摘要
以长寿花粉红色品种离体叶片为外植体,探讨了基本培养基、暗培养以及不同植物生长调节剂组合对不定芽再生的影响。结果表明,基本培养基以MS最为合适,以1/2MS+BA2.0mg/L+NAA0.1mg/L可以使粉红色品种的叶片不定芽的再生率高达5.2,暗培养15天可以将叶片的不定芽再生率提高到5.47。生根培养用1/2MS+IBA0.1mg/L最好。
The ideal regeneration system of Kalanchoe blossfeldiana with pink flower by leaf explants was established in this study, and the effects of basic medium, dark periods and plant growth regulators external on shoot regeneration from leaves of Kalanchoe blossfeldiana were investigated showed that the highest tratious on regeneration rate of 5.20 was obtained on the supplemented with 2.0 mg/L BA and 0.5 mg/L NAA .Two weeks of dark treatment could shoot regeneration rate to 5.47. MS supplemented with0.1mg/l IBA for rooting.
出处
《中国农学通报》
CSCD
2006年第5期79-81,共3页
Chinese Agricultural Science Bulletin
关键词
白花长寿花
组织培养
快速繁殖
Kalanchoe blossfeldianca Tissue culture,Pronagation
