摘要
玉米基因mir1编码一种抗秋季黏虫的半胱氨酸蛋白酶。利用RFLP作图mir1基因被定位在玉米第 6号染色体短臂上 ,但它在第 6号染色体短臂上的物理位置还不知道。实验以mir1和 4 5SrDNA为探针 ,通过双色荧光原位杂交技术确定了mir1基因在玉米细胞分裂中期和粗线期第 6号染色体上的物理位置。Southern杂交结果表明 ,在薏苡基因组中存在mir1基因的同源序列 ,进一步利用荧光原位杂交的方法确定mir1基因的同源序列定位于薏苡第 7号染色体长臂的近末端 ,其信号与着丝粒的百分距离为 73 33± 0 15。
The maize gene mir1 encoded a cystein proteinase which is resistant to fall armyworm.Previously,RFLP map indicated that the mir1 was mapped on chromosome 6.However,physical location of the mir1 gene on chromosome 6 has not been reported.In this study,the mir1 gene was physically located on the short arm of metaphase and pachytene chromosome 6 by dual-color fluorescence in situ hybridization (FISH) with 45S rDNA as a reference marker.The results of Southern blotting suggested that there were sequences homologous to mir1 in Coix lacryma-jobi L.genome.Then,the sequences were mapped on the distal region of long arm of chromosome 7 in C.lacryma-jobi by FISH.The percent distance from the signal site to centromere was 73.33±0.15.
基金
国家自然科学基金项目资助 (编号 :3 0 0 70 3 76)~~
关键词
mir1
玉米
薏苡
荧光原位杂交
mir1
maize
Coix lacryma-jobi L.
fluorescence in situ hybridization (FISH)
