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与Glh,Bph-3和xa-5连锁的BAC克隆在栽培稻和疣粒野生稻中的比较物理定位 被引量：1

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摘要 疣粒野生稻(O.granulata Nees et Arn ex watt)是分布于我国的3种最具研究和利用价值的野生稻资源之一.利用southern杂交和荧光原位杂交的方法,对水稻抗绿叶蝉基因Glh,抗褐飞虱基因Bph-3和抗白叶枯病基因xa-5在栽培稻(Oryza sativa L)和疣粒野生稻中的同源性和物理位置进行了比较分析.southern杂交结果表明,在疣粒野生稻中存在与水稻抗性基因连锁的RFLP标记的同源序列.RFLP标记筛选出来的3个BAC克隆怍专探针.在栽培稻和疣粒野生稻有丝分裂中期和减数分裂粗线期染色体中均检测到杂交信号.双色荧光原位杂交确定了3个BAC克隆中的2个(14E16和38J9)在疣粒野生稻同一对同源染色体的短臂上,且这2个克隆在栽培稻和疣粒稻染色体上还存在共线性.另一个BAC克隆(44B4)被定位到2个物种的另一染色体短臂末端.虽然栽培稻和疣粒野生稻的亲缘关系较远,在生物学特征和生态习性上有着极为明显的区别,但疣粒野生稻被检出的染色体在相对长度、臂比和BAC克隆在染色体上的相对位置与栽培稻的结果相似.
作者 熊志勇 谭光轩 游艾青 何光源 佘朝文 李立家 宋运淳
机构地区 武汉大学植物发育生物学教育部重点实验室 华中科技大学中-英联合实验室基因工程研究所
出处 《科学通报》 EI CAS CSCD 北大核心 2004年第3期252-257,共6页 Chinese Science Bulletin
关键词 水稻 疣粒野生稻 荧光原位杂交 比较物理定位 BAC克隆
分类号 S511.9 [农业科学—作物学]
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参考文献13

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二级参考文献14

共引文献287

同被引文献19

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