摘要
目的 了解徐州地区O15 7∶H7大肠杆菌宿主动物带菌情况及其毒力基因阳性率。方法 采集流行区内猪、鸡、羊、牛等家畜家禽粪便标本 ,用免疫磁珠法进行病原菌分离培养 ,并用多重引物PCR进行毒力基因分析。结果 共采集猪、鸡、羊、牛等家畜家禽粪便标本 92 5份 ,检出O15 7∶H7大肠杆菌 12 7株 ,检出率为 13 73%。其中 ,以牛、羊检出率较高 ,分别为18 6 0 %和 16 0 1%。对 4 2株菌株进行了SLT1、SLT2、eaeA和Hly四种毒力基因的检测 ,85 71%的菌株毒力基因阳性 ,且以同时带有SLT2、eaeA和Hly三种毒力基因最为常见 ,占带毒菌株的 80 5 6 %。病家分离的菌株带毒率 (96 4 3% )明显高于外对照 (6 4 2 9% ) (P <0 0 1)。结论 加强宿主动物O15 7∶H7监测 ,对疫情的分析和疫情预测具有重要意义。
Aim To investigate the O157∶H7 positive rate of host animal and the toxic gene of the strain in Xuzhou city,Jiangsu province.Methods Escherichia coliO157∶H7 was isolated with immune magnetic essay from feces of pig,chicken,sheep,cattle,and MPCR was performed to detect the toxic gene of the strains.Results Altogether 127 strains of O157∶H7 were separated from 925 feces of different animals ,the overall positive rate was 13.73%.The positive rates of cattle and sheep were high;it was 18.60% and 16.01% respectively.For 42 strains,SLT1,SLT2,eaeA and Hly toxic genes were detected,85.71% of the strain were,80.56% of them carried SLT2,eaeA and Hly genes simultaneously.The positive rate of virulence gene in strains detected from patient home was significantly higher than that of in the outer control.Conclusion It indicates that to enhance the surveillance of O157∶H7 in animal should be very important for the prediction and control of the disease.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2004年第4期318-320,共3页
Chinese Journal of Zoonoses
