摘要
用人乙型肝炎病毒的检测试剂,检测了成都地区6种家畜家禽的血清673份(只)。发现部分猪、兔和鸡、鹅血清HBsAg、HBeAg和^(32)P-HBV-DNA探针斑点杂交阳性,提示这些家畜家禽中可能存在类乙肝病毒的感染。还用免疫电镜在1份猪血清中观察到乙肝病毒样颗粒。
Using the reagent kits for detectinghuman HBV, primary serologic investi-gation was made in 6 kinds of domesticenimals and fowls (total 673) by ELISA,spot hybridization and immuno--electromi-croscope. The results showed that somesera samples of pigs, rabbits, chickenand geese were HBsAg, HBeAg positiveand positive for spot hybridization by us-ing ^(32)P-HBV-DNA probe, which suggestedthat HBV--like viruses might exist inthose domestic animals and fowls. HBV-like virus particles were seen in one pigserum sample which was HBsAg positive.The particles could be divided into threeforms in shape; large spherical particle(40-55nm), small spherical particle (20-30nm) and short filament particle. Thefurther studies are needed.
出处
《华西医科大学学报》
CAS
CSCD
1990年第2期181-182,共2页
Journal of West China University of Medical Sciences
关键词
家畜
家禽
类乙肝病毒
血清学
Domestic animals and fowls
HBV-like
virus
ELISA
Spothybridization
Immunoelectromicroscope
