丹参酮对阿尔茨海默病样大鼠学习记忆障碍的作用被引量：6

Effect of Tanshinone on Learning and Memory Impairment Induced by Amyloid β-peptide_ (1-40 )in Rats

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摘要目的探讨丹参酮 (Tan)对阿尔茨海默病 (AD)样大鼠学习记忆功能障碍的改善作用。方法采用凝聚态 β-淀粉样肽 (Aβ1-40 )在大鼠双侧海马齿状回背侧细胞带微量注射以建立拟人类AD样动物模型 ,灌胃给予AD样大鼠Tan (10 0 ,5 0mg/kg) ,采用明暗箱被动回避法、穿梭法检测Tan对大鼠学习记忆障碍的治疗作用。结果海马内注射Aβ1-40 (2 0 μg ) 14d后 ,经测试大鼠的学习记忆功能明显障碍 ,而给予Tan 10 0、5 0mg/kg可以不同程度的延长大鼠进洞潜伏期 (P <0 .0 1,P <0 .0 5 ) ,5 0mg/kg剂量组能较模型组显著增加大鼠主动回避次数 (P <0 .0 5 ) ,减少逃避失败次数 (P <0 .0 5 )。结论Tan对Aβ1-40 诱导大鼠的学习记忆功能障碍具有显著改善作用 ,Tan剂量在 5 0mg/kg时效果最好。 Objective To study the effects of Tanshinone (Tan) on learning and memory in rats with Alzheimer′s disease induced by amyloid β-peptide 1-40 (Aβ 1-40). Methods 10 μg incubated Aβ 1-40 was injected bilaterally into the dorsal blade of the dentate gyrus in the hippocampus of each rat. Two-compartment step-through passive avoidance test and shuttle-box active avoidance response test were used together to determine effects of Aβ 1-40 and that of Tan(100,50 mg/kg, ig) on learning and memory.Results Learning and memory dysfunction was detected by the behavioural test after Aβ 1-40 injection. After treatment with Tan, the latencies of passive avoidance test were markedly extended respectively in rats (P<0.01, P<0.05), and the number of avoidance was increased and escape failure of rats were decreased in Tan 50 mg/kg group (P<0.05, P<0.05). Conclusion Tan could obviously improve the impaired learning and memory induced by Aβ 1-40 in rats and the efficacy of Tan is better on the dosage of 50 mg/kg.

作者刘彤云李莉

机构地区咸宁学院药学院药学系咸宁学院医学院护理系

出处《咸宁学院学报（医学版）》 2004年第1期24-26,共3页 Journal of Xianning Univarsity(medical Sciences)

关键词丹参酮阿尔茨海默病大鼠学习记忆障碍药物治疗 Tanshinone Amyloid β-peptide Learning Memory Hippocampus

分类号 R277.791.6 [医药卫生—中医学] R285.5 [医药卫生—中药学]

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