摘要
目的:建立老年痴呆(Alzheimer's disease,AD)大鼠模型,探讨荔枝核皂苷对AD大鼠的治疗作用及其机制。方法:50只SD大鼠分为:对照组、模型对照组和低、中、高剂量荔枝核皂苷治疗组,共5组,每组10只;Morris水迷宫实验检测各组大鼠的逃避潜伏时间和平台跨越次数;Western blot实验检测各组脑组织β淀粉样蛋白(β-amyloid protein,Aβ)的表达变化;流式细胞仪检测各组脑组织活性氧(ROS)表达的改变。结果:AD大鼠模型组与对照组比较,逃避潜伏时间出现显著性地延长(P<0.01),平台跨越次数显著性地减少(P<0.01)。荔枝核皂苷治疗后AD大鼠的逃避潜伏时间和平台跨越次数得到显著性地改善(P<0.05)。AD大鼠脑组织Aβ的表达水平显著高于正常对照组,高、中、低剂量荔枝核皂苷治疗组可以剂量依赖性地降低模型鼠脑组织中Aβ的表达水平。对照组、模型对照组、低剂量荔枝核皂苷治疗组、中剂量荔枝核皂苷治疗组、高剂量荔枝核皂苷治疗组ROS表达水平的相对值分别为6.12±0.61、9.54±1.42、8.33±1.26、7.68±1.14、7.23±0.73,模型组显著高于对照组(P<0.01),荔枝核皂苷治疗可以显著降低模型组脑组织中ROS的表达水平(P<0.05)。结论:荔枝核皂苷具有抗AD大鼠痴呆的活性,减少AD大鼠脑组织Aβ和ROS的生成是其主要的作用机制。
AIM： To investigate the activity and mechanisms of lychee seed saponin against rats with senile dementia（AD）.METHODS： The 50 AD rats were randomly divided into control group,AD model group,low-dose lychee seed saponin group,medium-dose lychee seed saponin group,and high-dose lychee seed saponin group,respectively.Morris water maze examination was used to detect learning and memory ability.The protein expression was detected by Western blot analysis.The level of reactive oxygen species（ROS） was determined by flow cytometer analysis.RESULTS：The escape latency of rats was significantly increased in AD rats（P0.01）,and the numbers of rats crossing platform was significantly decreased in AD rats（P0.01）.The learning and memory ability were significantly developped by lychee seed saponin treatment.The protein expression of Aβ was significant higher in AD rats than that in normal control.The protein expression of Aβ was decreased in a dose-dependent manner by lychee seed saponin treatment in AD rats.The level of ROS in control group,AD model group,low-dose lychee seed saponin group,medium-dose lychee seed saponin group,and high-dose lychee seed saponin group was 6.12±0.61,9.54±1.42,8.33±1.26,7.68±1.14,and 7.23±0.73,respectively.The level of ROS was significant higher in AD rats than that in normal control.The level of ROS was decreased in a dose-dependent manner by lychee seed saponin treatment in AD rats.CONCLUSION： Lychee seed saponin showed the potent activity against AD rats.The decreasing of Aβ and ROS production may play a key role.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2012年第10期1098-1102,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
老年痴呆
荔枝核皂苷
Β淀粉样蛋白
活性氧
Senile dementia
Lychee seed saponin
β-amyloid protein（Aβ）
Reactive oxygen species
