摘要
AIM:To study the relationship between expression of plateletderived growth factor-BB (PDGF-BB) and fibrogenesis in chronic hepatitis B.METHODS:Hepatic tissues from 43 patients with chronic hepatitis B were embedded in paraffin.The sections were stained with HE and picric acid-sirius red to determine inflammatory activity and fibrosis stages. PDGF-BB expression was detected by immunohistochemistry and assessed semiquantatively. Levels of serum hyaluronic acid (HA),pro-collagen Ⅲ(PCⅢ), collagen Ⅳ (Ⅳ-C) and laminin (LN) were examined by radioimmunoassay (RIA).RESULTS:The expression level of PDGF-BB was found to be positively correlated with inflammatory activity, fibrosis stage and grade of histological findings (τ=0.58,0.55,0.55,P<0.01).The positive correlation was also observed between tissue level of PDGF-BB expression and contents of HA, PCⅢ, Ⅳ-C and LN in the circulation (r=0.52,0.32,0.40,0.33, P<0.05).CONCLUSION: PDGF-BB may play some role in the development and progression of liver fibrosis.
AIM:To study the relationship between expression of platelet- derived growth factor-BB (PDGF-BB) and fibrogenesis in chronic hepatitis B. METHODS:Hepatic tissues from 43 patients with chronic hepatitis B were embedded in paraffin.The sections were stained with HE and picric acid-sirius red to determine inflammatory activity and fibrosis stages.PDGF-BB expression was detected by immunohistochemistry and assessed semiquantatively.Levels of serum hyaluronic acid (HA), pro-collagen Ⅲ(PCⅢ),collagen Ⅳ(Ⅳ-C) and laminin (LN) were examined by radioimmunoassay (RIA). RESULTS:The expression level of PDGF-BB was found to be positively correlated with inflammatory activity,fbrosis stage and grade of histological findings (τ=0.58,0.55, 0.55,P<0.01).The positive correlation was also observed between tissue level of PDGF-BB expression and contents of HA,PC≡Ⅲ,Ⅳ-C and LN in the circulation (r=-0.52,0.32, 0.40,0.33,P<0.05). CONCLUSION:PDGF-BB may play some role in the development and progression of liver fibrosis.
