摘要
AIM:To observe the possible effects of transforming growth factor(TGF)β1,interleukin(IL)-6,tumor-necrosis factor(TNF)αand IL-10 on experimental rat hepatic fibrosis.METHODS:One hundred SD rats were divided randomly into the three groups.Control group received intraperitoneal injection of saline(2 ml-kg^-1),twice a week.Fibrogenesis group was injected intraperitoneally with 50%carbon tetrachloride(CCI4)(2 ml-kg^-1)twice a week.Fibrosisintervention group was given IL-10 at a dose of 4μg-kg^-120 minutes before CCI4 administration from the third week.At the fifth,seventh,and ninth weeks,7 to 10 rats in each group were sacrificed to collect serum.Levels of TGF-β1,TNF-α,IL-6 and IL-10 were determined by enzyme-linkedimmunosorbent assay(ELISA).The liver tissues were taken for routine histological examination.RESULTS:Hepatic fibrosis was developed with the injection of CCI4.Values of the circulating TGFβ,TNFα,IL-6 and IL-10 in the control group were 25.495.56 ng.L^-1,15.18±3.83ng.L^-1,63.64±13.03 ng.L-^1 and 132.90±12.13 ng.L^-1,respectively.Their levels in the CCI4-intoxication group were 31.13±6.41 ng.L^-1,18.91±5.31 ng.L^-1,89.08±25.39 ng.L^-1 and 57.63±18.88 ng.L^-1,respectively,and those in the IL-10-intervention group were 26.11±5.32 ng.L^-1,13.99±1.86 ng.L^-1,74.71±21.15 ng.L^-1 and 88.19±20.81 ng.L^-1,respectively.A gradual increase was observed in the levels of TGFβ1,TNFαand IL-6 during hepatic fibrogenesis.These changes were pardally reversed by simultaneous administration of IL-10.The histological parameters,characterized by CCl4-intoxificatJon,also seemed to be improved with IL-10 treatment,the collagen production was reduced at the ninth week and the histological activity index was decreased from 7.9±1.2 to 4.7±0.9.CONCLUSION:TGFβ1,TNFαand IL-6 may play important roles during CCI4-induced hepatic fibrogenesis,and IL-10 may counterbalance their effects.
基金
Supported by Science and Technology fund of Fujian Province,No.2003D05
肝纤维化
动物模型
细胞迁移
四氯化碳
慢性肝损伤
细胞因子
