摘要
'大矮蕉'MusaAAAcv.GrandeNaine的胚性细胞悬浮系(ECS)在M2液体培养基中预培养1周和2周后,分别将其接种在RD1或M3培养基上,于光照或黑暗条件下进行体胚的再生.再生体胚在接种后3周左右开始出现,经8周的培养后,在不同预培养时间、再生培养基种类及培养条件下,胚性细胞(团)质量增长了约5～18倍,从沉积细胞体积(SCV)为1mL的ECS获得的再生体胚数为0 71×105～3 07×105.植株的再生率及从SCV为1mL的ECS获得的再生植株数也因上述体胚再生条件的不同而异.
Embryogenic cell suspensions (ECS) of 'Grande Naine' (Musa AAA group) were plated on RD1 or M3 medium for the regeneration of somatic embryos, 1 to 2 weeks after last subculture. The first regenerable somatic embryos were observed about 3 weeks after inoculation. After 8 weeks of culture, the mass of the embryogenic mass increased about 5 to 18 times and the number of somatic embryos that could be regenerated from 1 mL settled cell volume (SCV) of ECS ranged between 0.71×10~5 and 3.07×10~5, depending on pre-culture time in liquid medium before regeneration, regeneration media and incubation conditions (light/dark). The frequency of plant recovery and the amount of plantlets regenerated from 1 mL SCV of ECS were indirectly affected by somatic embryos regeneration conditions that were studied.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2004年第2期63-66,共4页
Journal of South China Agricultural University
基金
国家自然科学基金资助项目(39870434)
广东省自然科学基金资助项目(990714)
广东省科技攻关资助项目(C20209)
关键词
体胚发生
大矮蕉品种
植株再生
胚性细胞悬浮系
培养基
香蕉
banana (Musa spp.)
embryogenic cell suspension
somatic embryogenesis
regeneration of plants
