摘要
目的 观察大鼠失神经支配骨骼肌组织学、电生理及酶组织化学改变 ,探讨反映肌肉萎缩程度的指标。方法 选用SD大鼠 ,建立失神经支配腓肠肌模型 ,观察术后肌细胞直径及截面积 ,运动终板超微结构、纤颤电位波幅、Na+ 、K+ ATP酶及Ca2 + ATP酶活性变化。结果 肌细胞直径及截面积随失神经支配时间延长呈进行性下降 ;运动终板 4周内改变不明显 ,16周后消失 ;纤颤电位波幅在 8周内维持在高水平 ,12周后呈进行性下降 ;Na+ 、K+ ATP酶活性随失神经支配时间延长呈进行性下降 ;Ca2 + ATP酶活性在 8周内维持在较高水平 ,12周后明显下降。结论 肌细胞直径及截面积可作为反映肌肉萎缩的可靠形态学指标 ,纤颤电位波幅可作为潜在电生理指标 ;Na+ 、K+ ATP酶活性是可靠酶组织化学指标。
Objective To study the histology,electro-physiology and enzymic histochemistry of denervated skeletal muscle to find out the index evaluating the degree of muscle atrophy.Methods Forty-eight SD rats were used and the model of denervated skeletal muscle was made by cutting off the right tibial nerve.The myocyte diameter and cross section area,motor end plate,fibrillation potential amplitude,Na +,K +-ATPase and Ca 2+ -ATPase activities were observed.Results The myocyte diameter and cross section were reduced progressively along with the duration of muscle denervation.The change of motor end plate was not significant within 4 weeks and disappeared after 16 weeks.The fibrillation potential amplitude could remain at a higher level within 8 weeks and reduced progressively afterwards.Na +,K +-ATPase activity was reduced progressively along with the duration of denervation.Ca 2+ -ATPase activity could maintain at a higher level within 8 weeks and was reduced significantly afterwards.Conclusion The myocyte diameter and cross section area was a credible morphologic index of muscle atrophy.The fibrillation potential amplitude could be considered as a potential electric physiologic index.Na +,K +-ATPase-ATPase activity was a credible enzymic histochemisty index.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2004年第1期57-58,共2页
Chinese Journal of Experimental Surgery
基金
上海市医学领先学科基金资助项目 (95 Ⅲ 0 0 1 )
关键词
大鼠
骨骼肌萎缩
实验研究
运动终板
纤颤电位
Skeletal muscle
Motor end plate
Fibrillation potential
Na+,K+-ATPase and Ca2+-ATPase
Ca2+-ATPase
