摘要
用pAW1015kb的DNA片段为探针检测人流胚胎与其亲代的DNA限制性片段长度多态性(RFLPs)的遗传关系。同时测定他们的某些同工酶(葡萄糖磷酸变位酶、酯酶D、乙二醛酶Ⅰ)型。在不违反孟德尔遗传规律的前提下,按M(?)ller-Essen公式计算,其父权概率都已达到或超过可以确认亲生关系的标准。这一方法的建立,使过去棘手的早期妊娠的亲权鉴定之难题获得圆满解决。此外,本文还对这一方法的优越性进行了讨论。
The results of determining the genetic relationship between restriction fragment length polymorphisms (RFLPs) of aborted feta and their parents, using pAW101 5 kb DNA fragment as a probe, were presented. The exclusion probability of paternity, using this technique, reached 91.4%, it was equal to the level of other techniques (including HLA-A, -B typing) used presently. When the genetic patterns of PGM1 subtype, EsD, GLO I isozymes were detected at the same time, the exclusion probability of paternity could be increased to 94.23%. The paternity probabilities of all non-disputed cases tested had reached the standards of including paternity, provided that there is no inconsistency with the Mendelian Law. The establishment of this technique has solved the problem of determining the paternity of fetus within the first trimester of gestation. And the advantages of this technique has been discussed.
出处
《中山医科大学学报》
CSCD
1992年第3期40-43,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
国家自然科学基金资助项目
关键词
胚胎
亲权鉴定
DNA探针
人工流产
restriction fragment length polymorphisms(RFLPs)
aborted fetus
paternity testing
DNA probe
