摘要
根据随机探针检测DNA限制片段长度多态性的原理和人与鼠的髓鞘硷性蛋白(MBP)基因cDNA有90％以上同源序列的事实,我们选用rMBP-cDNA 3'端非表达区高度重复顺序的0.81kb片段作探针,检测用HaeⅢ酶解的人DNA限制性片段结果可以分解出22条谱带,受检的30例无血缘关系的个体之间,没有两个人的谱带是完全相同的,显示出此方法的高度特异性。本文还比较了若干DNA片段作探针和几种限制性内切酶检测人DNA指纹的结果。
Basing on the principle of detecting restriction fragment length polymorphisms (RFLPs) With anonymous DNA probes and the fact that there were more than 90% homologeous sequences in the myelin basic protein (MBP) gene between man and rat, we used the 0. 81kb fragment in highly repeat sequence of the 3'-end non-coding region of rMBP-cDNA as a probe to detect RFJLs of human DNA digested with HaeⅢ. 22 bands were found on the autoradiographes. The identical autoradiographes between two unrelated persons was not found the DNA finger-prints obtained is highly specific. The DNA fingerprints obtained by several DNA probes and restriction endonucleases were compared.
出处
《中国法医学杂志》
CSCD
1989年第4期207-209,共3页
Chinese Journal of Forensic Medicine
基金
国家自然科学基金
关键词
DNA指纹
限制性片段
长度多态性
DNA fingerprint Restriction length polymorphismsl (RFLPs)
