摘要
在文蛤(Meretrix Meretrix)体内具有血球凝集活性的凝集素(简称MML),经实验证实仅分布于文蛤外套腔液中。向外套腔液中加入固体硫酸铵达40％时,可使99％的MML沉淀。用不同饱和度的硫酸铵溶液依次对上述沉淀进行抽提,得到MML粗制品溶液,其比活比原始外套腔液提高了约9.95倍,总活力回收达48％。最后用自制的人血浆糖蛋白一Sepharose 4 B亲合层析柱可将上述MML粗制品的比活再提高400多倍。结果与原始外套腔液比较,MML终产品的比活总共提高了4313倍,总活力回收达44.8％,在电泳鉴定时显示出单一的区带。
A lectin (shortly call MML) with high human blood red cell agglutinat-ion activity was detected in the sea clam (Meretrix Meretrix). This lectin(MML)distributes only in the mental cavity fluid of the sea clam. When adding solid ammonium sulfate into the mental cavity fluid upto 40% concentration at 4℃,99% of the total activities of MML were precipitated. The precipitate was con-tinually extracted by cold ammonium sulfate with different concentration. The last supernatant which was called primary MML fluid gived a 9.95-fold purifica-tion and a 48% recovery of total activities compared with the crud mental cavi-ty fluid. Finally by using our self-made human plasma glycoprotein-Sepharose 4B affinity colum chromatography, the MML in the primary MML fluid was highly purified. Compared with the crud mental cavity fluid the final purified MML gived a 4 313-fold purification and a 44.8% recovery of total activities.This final purified MML exhibted one band on plate alkaline disc-PAGE.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
1992年第1期86-92,共7页
Acta Scientiarum Naturalium Universitatis Sunyatseni
关键词
凝集素
文蛤
沉淀
抽提
硫酸铵
ammonium Sulfate
precipitate
extract
MML (lectin from sea clam Meretrix Meretrix)
human plasma glycoprotein-Sepharose 4B
affinity
