摘要
取不同发育阶段文蛤 (MeretrixmeretrixLinnaeus)活体解剖取出生殖腺 ,常规方法制成切片 ,以透射电镜(TEM )观察其卵母细胞的卵黄发生过程。结果表明 ,文蛤的卵母细胞卵黄发生期间 ,线粒体、内质网、高尔基复合体、溶酶体、微吞饮泡等细胞器均参与了卵黄粒的形成。卵黄合成早期的卵母细胞质膜伸出大量的微绒毛 ,并出现卵黄膜 ,胞质中有大量膜性小泡 ,溶酶体、线粒体、高尔基复合体和粗面内质网发达 ,卵黄前体物质逐渐增多。在卵黄合成中期 ,胞质内线粒体和内质网活动活跃 ,卵母细胞大量合成和积累卵黄物质 ,细胞质膜外凸 ,与外界进行物质交换。卵黄合成后期 ,卵质内贮存了大量的卵黄粒 ,细胞器不发达。此外 ,还对卵母细胞发育过程卵黄颗粒的细胞内。
The gonad samples were collected from Meretrix meretrix at different development stages in vivo,and using routine method the slices were made. Under transmission electron microscope the ultrastructure of vitellogenic oocytes was observed. The results show that ,at the stage of vitellogenesis,the organelle,i.e. mitochondria, endoplasmic reticulum,Golgi complex,lysosome,micropinocytes vesicle and so on,is involved in the formation of yolk granules. At the early stage of vitellogenesis,a large amount of microvillie grow up and vitelline membranes appear; a lot of micropinocytes vesicle forms in the oocyte cytoplasm; the lysosome, mitochondria,Golgi complex and the rough endoplasmic reticulum are developed; the yolk precursor is increasing in number. At the mid stage of vitellogenesis,well developed mitochondria and endoplasmic reticulum present in the cytoplasm and a lot of yolk granules form and accumulate in the oocyte; the evagination of the cytoplasmic membrane shows convex and leads to exchange the materials in the external environment. At the end stage,a large number of yolk bodies fill in the cytoplasm and the organelles are not developed. Also,a discussion is made on the raw materials from intracell or extracell for the formation of vitellogenesis during the development of oocytes.
出处
《中国水产科学》
CAS
CSCD
北大核心
2002年第2期125-128,共4页
Journal of Fishery Sciences of China
