摘要
目的:研究颈交感神经干离断对浸水大鼠胃黏膜血流量的影响. 方法:30只♂SD大鼠随机分为3组(n=10)Ⅰ组为正常对照组,Ⅱ组为浸水对照组;Ⅲ组为颈交感神经千离断后浸水组. Ⅰ、Ⅱ组只将颈交感神经干暴露、分离,不离断;Ⅲ组离断颈交感神经干并将断端结扎.将Ⅱ、Ⅲ组大鼠垂直浸水(水温23±1℃)至剑突水平,6h后取出,测定各组胃黏膜血流量(GMBF),评价黏膜损伤程度.放免法测定血浆及胃黏膜ET-1含量. 结果:Ⅱ组胃黏膜见出血及糜烂.Ⅲ组黏膜损伤程度明显轻于Ⅱ组,Ⅲ组与Ⅱ组比较GMBF明显升高,二者分别为,130.0±14.5;68.9±12.7(P<0.01).Ⅱ组和Ⅲ组的溃疡指数(UI)分别为50.1±12.3;26.6±9.4差异极显著(P<0.01),Ⅱ组和Ⅲ组的GMBF值与UI呈高度负相关,γ=-0.847, P<0.001.Ⅱ组大鼠的血浆及胃黏膜的ET-1值明显高于Ⅰ组,P<0.01;Ⅲ组的血浆ET-1值也明显高于Ⅰ组但与Ⅱ组比较明显减少,P<0.01.Ⅲ组大鼠的胃黏膜组织的ET- 1值明显高于Ⅰ组,P<0.01,但与Ⅱ组比较无显著差异. 结论:颈交感干离断可增加浸水应激大鼠胃黏膜血流量, 对胃黏膜损伤有保护作用.血浆及胃黏膜ET-1参与了颈交感神经干离断对胃黏膜保护的作用.
AIM: To investigate the effect of transections of the cervical sympathetic trunk (TCST) on the gastric mucosal blood flow (GMBF) in water immersion restrain rats. METHODS: A total of 30 male Sprague-Dawleys rats were randomly allocated into three groups: control group (Group Ⅰ), water immersion restrain group (Group Ⅱ) and TCST followed by water immersion restrain (Group Ⅲ). Superior cervical ganglion were exposed and splited, but not cut off in Group Ⅰ and Group Ⅱ. In Group Ⅲ, we cut off and ligated the cervical sympathetic nerve trunk below 3 mm of superior cervical ganglion. Then the rats of Group Ⅱ and Group Ⅲ were vertically immersed into water (23±1℃). Six hours later, GMBF were measured by laser Doppler blood stream meter. And we evaluated the gastric mucosal ulcer index (UI) according to Guth score. RESULTS: There was mucosal bleeding and erosion in Group Ⅱ, Group Ⅲ was obviously relived as compared with Group Ⅱ. The GMBF was 68.9±12.7; 130.0±14.5 (PU) in Group Ⅱ and Group Ⅲ respectively, while the UI was 50.1± 12.3; 26.6±9.4.(P<0.01). The GMBF and UI was significant negative correlation (γ = -0.847, P<0.01). ET-1 in plasma and gastric mucosa in Group Ⅱ were higher than those in Group Ⅰ. ET-1 in plasma in Group Ⅲ were higher than that in Group Ⅰ, while they were lower than that in GroupⅡ. ET-1 in plasma had a moderate negative-correlation with GMBF. CONCLUSION: Increased the GMBF due to TCST may contribute to attenuation in the lesion of gastric muscoa in water immersion restrain rats, and it may have something to do with ET-1 in plasma and gastric mucosa.
出处
《世界华人消化杂志》
CAS
2004年第4期907-910,共4页
World Chinese Journal of Digestology
