摘要
目的 从生化及形态学角度研究应激性溃疡 (SU)发生发展过程中胃黏膜细胞的死亡形式及增殖功能的变化。方法 原位末端标记 (TUNEL)法检测水浸 束缚应激 (WRS)结束前后不同时间点细胞凋亡的发生 ;透射电镜下观察细胞形态的变化 ;免疫组化ABC法原位检测增殖细胞核抗原(PCNA)蛋白的表达。结果 正常大鼠胃黏膜上皮散在TUNEL阳性细胞 ,PCNA蛋白主要在胃腺颈部近胃小凹处呈中等阳性表达。WRS 2h～WRS结束后 5hTUNEL阳性细胞较对照组明显增多 (P <0 .0 1)并逐渐达高峰 ,而PCNA表达较正常对照组显著减少 (P <0 .0 1) ;WRS后 8～ 12hTUNEL阳性细胞逐渐减少 ,而PCNA表达达高峰 ;WRS后 2 4hTUNEL阳性细胞仍高于正常水平 (P <0 .0 5 ) ,PCNA表达基本恢复至正常水平 (P >0 .0 5 )。透射电镜下可见正常胃黏膜细胞形态规则 ,核形规整 ;而发生凋亡的细胞形态各异。结论 应激期胃黏膜细胞凋亡明显增加 ,增殖能力下降 ,它们之间的失衡是溃疡发生的重要原因 ;在应激性损伤恢复的早期阶段 ,大量细胞发生凋亡可能是启动细胞增殖的必要条件。
Objective To investigate the gastric epithelial cell death and proliferation in water restraint stress (WRS) rats during the processes of pathogenesis of stress ulcer, by means of biochemical and morphological analysis. Methods Apoptotic cells were quantitated in gastric mucosa before or at different time points after WRS by TUNEL techniques, and the morphological changes of cells were observed by transmisson electronic microscope whilst the cell proliferation was detected by immunohistochemical staining for proliferating cell nuclear antigen (PCNA). Results The result revealed that in normal controls, the apoptotic cells were seen only occasionally at the surface epithelium,and the PCNA positive cells were found in the proliferative zone of the gastric glands. In contrast, the apoptotic quantities rose significantly from 2 h after the WRS to 5 h after the termination of WRS, and reached a peak gradually,while PCNA positive cells decreased significantly. At 8～12 h after the WRS ,the apoptotic cells gradually declined but failed to return to normal even at 24 h. The PCNA cells increased to reach a peak and declined at 24 h towards the value observed in the intact rats. Under the transmission electronic microscopy it showed that in contrast to normal mucosal cells, the morphology of apoptotic cells changed with various characteristics. Conclusions The results suggested that the epithelial apoptosis significantly increased, whereas proliferation decreased; the imbalance between both them induced the damage of gastric mucosal integrity. During the initial stage of mucosal recovery from stress lesion, the apoptosis appears to activate the mucosal cell proliferating, a response leading to the recovery of the mucosa from the stress damage.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2003年第10期595-598,共4页
Chinese Journal of Digestion
基金
"十五"全军医药科研基金重点项目 (0 1Z0 5 9)
