摘要
目的:研究肿瘤转移抑制基因KAI1对MHCC97-H细胞裸鼠成瘤及转移的影响. 方法:将已转染KAIl正、反义核苷酸的高转移潜能肝癌细胞MHCC97-H接种裸鼠皮下,观察皮下肿瘤生长情况, 再将皮下肿瘤组织进行裸鼠原位肝接种,观察接种后肿瘤肺转移情况.其中,转染空载体及未转染的MHCC97-H亲本细胞作为实验对照组. 结果:正义组、反义组、空载体组及亲本细胞组裸鼠皮下均成瘤,肿瘤出现的时间、肿瘤块生长的速度无明显差异, 生长方式上反义组表现出明显的侵袭性.原位肝接种6 wk 后裸鼠肺部均出现癌巢,AFP染色可见癌巢中肿瘤细胞质有黄色颗粒沉着,证实为肝癌转移至肺形成的转移灶.与MHCC97-H亲本细胞组比较,反义组转移灶数目明显增加(P=0.00158),正义组转移灶数目明显减少(P=0.00465),差异均有显著性,而载体组传移灶数目无明显变化(P=0.15166). 结论:肿瘤转移抑制基因KAI1对MHCC97-H细胞裸鼠成瘤性及肿瘤生长无明显影响,但上调KAI1蛋白的表达水平能在一定程度上降低肿瘤的侵袭性、抑制转移的发生.这为肝癌的抗转移治疗研究提供了重要线索.
AIM: To explore the effects of metastasis-suppressor gene KAI1 on tumorigenicity and metastasis of hepatocellular carcinoma cells MHCC97-H with high metastatic potential in nude mice. METHODS: The MHCC97-H hepatocellular carcinoma cells with high metastatic potential transfected with sense or antisense KAI1 expression plasmid in our previous experiments were inoculated subcutaneously into nude mice. The growth of subcutaneous tumor was observed, then the subcutaneous tumor tissues were harvested and implanted orthotopicly into nude mice liver. The tumor metastasis in lung was carefully examined under microscope. MHCC97-H and the cells transfected with vector pCI-neo, but without KAI1 gene were used as control. RESULTS: The different cells inoculated subcutaneously all had tumorigenicity. There were no significant differences in the speed of tumor growth among different groups, but the antisense group showed stronger invasion ability than others. The spontaneous metastasis to lung occurred in latency period of six weeks via orthotopic implantation of tumor tissue. Immunostaining showed strongly positive for AFP in lung metastatic lesions, which indicated those were lung metastatic lesions from hepatocellular carcinoma. The number of lung metastatic lesions increased dramaticly in antisense group (P=0.00158), decreased significantly in sense group (P=0.00465) and no significant difference in vector pCI-neo group (P=0.15166), as compared with their paternal MHCC97-H group. CONCLUSION: The metastasis-suppressor gene KAI1 has no significant effects on tumorigenicity and growth of MHCC97-H hepatocellular carcinoma cells, but enhanced KAI1 expression can decrease the invasion ability and inhibit the metastasis, which offers an important clue to investigate the anti-metastasis treatment for hepatocellular carcinoma.
出处
《世界华人消化杂志》
CAS
2004年第4期778-781,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30070348~~
