摘要
目的 研究肠道非霍奇金淋巴瘤的临床病理、免疫表型及与EBV感染的相关性。方法 应用多种抗体标记B淋巴细胞 (CD2 0 ,CD79a ,CD4 5RA ,κ ,λ ,CD5 ,CD10 ,bcl 2 ,cyclinD1)和T淋巴细胞 (CD3ε,CD8,CD4 5RO ,CD5 6 ,GramB ,TIA) ,同时进行细胞增殖活性的检测 (Ki 6 7)、免疫组织化学染色 (SABC法 )、EBV寡核苷酸探针 (EBER)原位杂交。结果 6 0例中 ,免疫组化证实B细胞性淋巴瘤 4 8例 (80 % ) ,T细胞性淋巴瘤 12例 (2 0 % )。EBV EBER原位杂交 4 0例中 6例阳性表达 ,均为T细胞性淋巴瘤 ,阳性细胞占肿瘤细胞的 30 %～ 80 % ,34例B细胞性淋巴瘤EB病毒检测阴性。结论 肠道淋巴瘤以B细胞淋巴瘤多发 ,并以惰性的黏膜相关性边缘区B细胞性淋巴瘤多发 ,与EBV无相关性。肠道T细胞性淋巴瘤侵袭性较强 ,且与EBV相关性较高。
Objectives To study the histological subtype,immunophenotype of non-Hodgkin lymphoma of intestine and relationship with Epstein-Barr virus (EBV) infection. Methods Immunohistochemical studies(SABCmethod) were performed using monoclonal and polyclonal antibodies marked B-cell (CD30,CD79a,CD45RA,κ,λ,CD5,CD10,bcl-2,cyclin D1),T-cell(CD3ε,CD8,CD45RO,CD56,gram B,TIA),and cell proliferation marker(Ki-67);For differentiatal diagnosis between Hodgkin lymphoma and non-lymphocytic neoplasm,immunohistochemistry was used (CD20,CD68,CD21,EMA,SMA,CD15,CD30) for immunophenotype,cell differentiation and hyperplastic state. Insitu hybridization for EBV encoded small nuclear RNA(EBER) was used to detect the EBV infection in the lymphoma of intestine. Results Most of 60 cases of non-Hodgkin lymphomas in the intestine located at the lower portion of small intestine and colon,and single mass was more often to be seen with ulcer on the surface.Of the 60 cases,12 (20%)and 48(80%)cases were confirmed as T cell and B cell lymphomas respectively,using immunohistochemical method.According to WHO classification,T cell lymphoma cases were peripheral T (12/60 cases,20%).B cell lymphomas were diffuse large B cell (12/60 cases,20%), marginal B cell lymphomas (36/60 cases,60%,MALT type).EBV-EBER in situ hybridization was positive in 6/40 cases(15%),all of which were T cell type.There were about 30%～80% tumor cells positive.There was no positive cell in B cell lymphomas. Conclusion The studis shows that the lymphomas of intestine are more often as B cell type and most of them were indolent in clinical symptom (low degree malignancy),which suggests that T cell lymphoma always means invasive (higher malignant).Our results also show that T cell lymphoma has a close relationship with EBV infection, but B cell type is not.
出处
《诊断病理学杂志》
CSCD
2003年第6期336-338,共3页
Chinese Journal of Diagnostic Pathology
基金
( 98 5 )医学创新人才培养工程基金资助项目 ( 2 0 0 1-69-87)
