摘要
目的:探讨大肠癌组织VEGF-C和Flt-4的表达与淋巴管计数及病理参数的关系。方法:采用酶组织化学方法行淋巴管染色;RT-PCR方法检测VEGF-C及Flt-4的表达。结果:VEGF-C和Flt-4的表达密切相关(P<0.05),VEGF-C阳性淋巴管数较VEGF-C阴性淋巴管数明显增多(25.16±7.52;17.14±7.22)(P<0.01)。Flt-4阳性淋巴管数较Flt-4阴性淋巴管数明显增多(25.34±7.22,18.93±8.30)(P<0.05)。两者表达均与肿瘤淋巴结转移有关。结论:VEGF-C通过激活Flt-4诱导大肠癌间质中淋巴管增生,为肿瘤细胞向淋巴管转移提供了条件。
Objective: There may be a close relationship among the expression of VEGF-C and its receptor Flt-4 and lymphangiogenesis and lymph node metastasis in malignant tumor. The purpose of this study was to comparethe relationship among VEGF-C mRNA espression, Flt-4 mRNA expression, density of lymphatic vessles, and pathology parameters in colorectal cancer. Methods: Lymphatic vessles were stained by enzyme histochemical method. VEGF-C mRNA and Flt-4 mRNA were determined by RT-PCR. Results: There was a statistically positive correlation between the incidence of VEGF-C and Flt-4 mRNA expression in colorectal cancer (P<0.05). The number of lymphatic vessels in VEGF-C mRNA positive tumors was significantly larger than that in VEGF-C negative tumors (27.61±5.80, 18.12±6.38) (P<0.05), similarly, the number of lymphatic vessels in Flt-4 mRNA positive tumors was larger than that in Flt-4 negative tumors (25.34±7.22,18.93±8.30)(P<0.05). Both the expression of VEGF-C and Flt-4 were closely related to the lymph node metastasis. VEGF-C expression were related to the depth of tumor invasion, but the expression of Flt-4 were related to the grade of tumor differentiation. Conclusion: VEGF-C may induce the proliforation of lymphatic vessels in the stroma of primary colorectal cancer via activation of Flt-4. As a result, cancer cells can easily invade the lymphatic vessel.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2004年第6期319-321,共3页
Chinese Journal of Clinical Oncology
基金
河北省科技攻关基金资助(编号:022761031~01)
