摘要
目的研究肺腺癌组织中血管内皮生长因子C(VEGF-C)、血管内皮生长因子D(VEGF-D)蛋白表达与微淋巴管密度(MLVD)及淋巴结转移之间的关系,探讨癌周淋巴管生成的机制。方法采用逆转录聚合酶链法(RT-PCR),检测VEGF-C、VEGF-D在48例肺腺癌新鲜组织中的表达;采用免疫组织化学法检测肺腺癌组织周边和内部VEGF-C、VEGF-D和MLVD蛋白的表达。结果VEGF-C和VEGF-D mRNA在肺腺癌组织中高表达,在正常肺组织低表达(P<0.01);VEGF-C、VEGF-D蛋白阳性率分别为70.8%(34/48)和58.3%(28/48),其中肿瘤周边部位的表达显著高于肿瘤中心部位,差异有统计学意义;在VEGF-C、VEGF-D蛋白阳性组,MLVD显著高于阴性组(P<0.01),淋巴结转移发生率增加(P=0.042,P=0.012)。结论VEGF-C和VEGF-D的表达可诱导肺腺癌癌周淋巴管的生成,促进肿瘤的经淋巴道转移。
Objective To investigate the relationship among vascular endothelial growth factor -C, （VEGF-C）, VEGF-D, microlymphafic vessel density （MLVD） and lymph node metastasis in lung adenoearcinoma, and to explore the mechanism of cancereus peripheral tissue lymphatic vessels. Methods The expressions of VEGF-C and VEGF-D mRNA were determined by the semi-quantitative reverse transcriptase-polymerase chain reaction method and the expressions of VEGF-C, VEGF-D and MLVD were determined in the center of the tumors and peripheral tissues by means of irranunohistoehemistry in 48 cases of lung adenocarcinoma. Results Expressions of VEGF-C and VEGF-D mRNA were higher in lung cancer than in normal lung tissues, positive ratio of VEGF-C and VEGF-D was 70.8% （34/48） and 58.3（28/48）. The expressions of VEGF-C and VEGF-D in cancerous peripheral tissues were significantly higher than those in the center of the tumors. The MLVD and positive lymph node metastasis in the positive VEGF-C or VEGF-D group were higher than those in the negative group. Conclusion The expression of VEGF-C and VEGF-D can induce lymphangiogenesis of lung adenocarcinoma and lymph metastasis.
出处
《山东大学学报（医学版）》
CAS
北大核心
2008年第6期616-619,共4页
Journal of Shandong University:Health Sciences
基金
山东省自然科学基金资助项目(2005ZRB14159)
关键词
肺肿瘤
血管生成因子
淋巴结
Lung neoplasms
Angiegenesis factor
Lymph nodes
