摘要
目的 :探讨睾丸支持细胞免疫豁免功能发生的机制。方法 :分离培养 4 8h后的睾丸支持细胞与活化的淋巴细胞共同培养 ,MTT法检测其对淋巴细胞的杀伤作用 ;应用抗FasIgG抗体阻断试验评价睾丸支持细胞杀伤活化的淋巴细胞与Fas/FasL系统之间的关系。结果 :睾丸支持细胞体外可以杀伤活化的淋巴细胞 ,该种杀伤作用可以被抗FasIgG抗体阻断 ,表明睾丸支持细胞通过Fas/FasL系统发挥其免疫豁免功能。结论 :睾丸支持细胞能通过Fas/FasL作用而调节T细胞的活性 ,这可能是睾丸支持细胞提供局部免疫豁免功能的机制之一。
Objective:To investigate the mechanism of immune privileged origin from sertoli cell.Methods:The sertoli cells were cultured together with active lymphocytes to observe the killing effect on lymphocytes by MTT; and the Fas/FasL system was blocked by anti Fas IgG antigen so as to reveal the mechanism of this killing effect.In the meantime,the expression of FasL in sertoli was tested.Resrlts:Sertoli cells can kill active lymphocytes and express the Fas ligand.This killing effect can be blocked by the anti Fas antiboby.It suggested that the killing effect was mediated by Fas/FasL system.Conclusion:Sertoli cells can regulate the activtity of lymphocytes and the FasL expression on sertoli cells may be the mechanism of immune privilege.
出处
《中国现代普通外科进展》
CAS
2004年第1期23-25,共3页
Chinese Journal of Current Advances in General Surgery
基金
四川省科技厅科研基金资助项目 (0 2SY0 2 9 165)
