摘要
目的 探讨细胞增殖标记物 (PCNA、ki 67)在乳腺癌中的表达及与乳腺癌预后的关系。方法 应用免疫组织化学法EnVision法染色 ,检测 112例乳腺癌原发灶PCNA(单克隆抗体PC10 )的表达 ,计数MIB1标记指数 (MIB1Labellingindex ,MIB1LI) ,同时根据腺管形成、核的多形性及核分裂像数确定的分级标准、舟山会议推荐方案和TNM国际分期标准 ,对 112例乳腺癌进行组织学分类、分型、分级和分期 ,收集临床资料并进行随访。结果 112例中肿瘤直径超过 2cm的患者、临床分期为Ⅲ～Ⅳ期的患者、腋窝淋巴结有转移的患者和腋窝淋巴结受累个数 4个以上的乳腺癌患者的MIB1LI、PCNA高表达的比率均分别显著高于肿瘤直径不足 2cm的患者、临床分期为Ⅰ～Ⅱ期的患者、腋窝淋巴结无转移的患者和腋窝淋巴结受累个数少于 3个 (含 3个 )的乳腺癌患者 (P <0 0 5 ) ,而前者的 10年累积生存率均分别低于后者 ;患者年龄和月经状况与MIB1LI、PCNA表达强度及 10年累积生存率无显著性差异 (P >0 0 5 )。结论 MIB1LI、PCNA表达强度与乳腺癌肿瘤直径、临床分期、腋窝淋巴结状况及腋窝淋巴结受累个数显著影响MIB1LI、PCNA的表达强度 ,是判定乳腺癌预后的可靠和重要的指标之一。
Objective To explore the relationship between the expressions of cell proliferation markers(PCNA and Ki 67) and prognosis of breast carcinoma. Methods The expression of proliferating cell nuclear antigen(PCNA)(monoclonal antibody PC10) in 112 cases of primary breast carcinoma was detected by immunohistochemical method(EnVision staining). MIB1 labelling index(MIB1 LI) in the breast carcinoma was detected. Histological classification, typing, grading and staging of 112 cases of breast carcinoma were conducted on the basis of the reported diagnostic criteria. The clinical data were collected and a follow up was conducted. Results Expressions of MIB1 LI and PCNA in patients with tumor larger than 2 cm in size, at Ⅲ-Ⅳ, with metastasis in axillary lymph nodes, or with more than four axillary lymph nodes involved were significantly higher than those in patients with tumor less than 2 cm in size, at Ⅰ-Ⅱ, without metastasis in axillary lymph nodes, or with no more than 3 axillary lymph nodes involved( P <0 05). The cumulative 10 year survival rate of the former was lower than that of the latter. Ages and menstrual conditions were not significantly related with expression levels of MIB1 LI and PCNA and cumulative 10 year survival rate( P >0 05). Conclusion Expression intensity of MIB1 LI and PCNA, closely associated with many clinical and pathological factors, may be the reliable and important factor for judging the prognosis of breast carcinoma.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第23期2097-2100,共4页
Journal of Third Military Medical University
关键词
乳腺肿瘤
组织学
预后
增殖细胞核抗原
breast carcinoma
histology
prognosis
proliferating cell nuclear antigen
