摘要
目的 检测基质金属蛋白酶 9(MMP 9)及其抑制物 1 (TIMP 1 )mRNA在原因不明性不孕患者黄体中期子宫内膜中的表达 ,及与性激素的相关性。方法 采用原位杂交、逆转录聚合酶链反应(RT PCR)技术 ,对 38例原因不明不孕患者 (研究组 )和 2 0例正常生育期妇女志愿者或男方因素不孕患者 (对组照 )的黄体中期子宫内膜行MMP 9、TIMP 1mRNA检测 ,同期放射免疫法测定雌二醇 (E2 )、孕酮 (P)水平。结果 子宫内膜腺上皮、间质细胞胞浆、胞核均有MMP 9、TIMP 1mRNA的表达 ,研究组较对照组表达少 ,研究组黄体中期子宫内膜MMP 9mRNA表达水平为 0 .42± 0 .1 9,显著低于对照组的 0 .57± 0 .1 9(P <0 .0 5) ;研究组TIMP 1mRNA表达水平为 0 .59± 0 .1 9,显著低于对照组的 0 81± 0 .2 0 (P <0 .0 1 )。研究组黄体中期血清P水平为 (34± 1 5)nmol/L ,显著低于对照组的 (53± 1 7)nmol/L(P<0 .0 1 ) ,而血清E2 水平与对照组比较 ,差异无显著性 (P >0 .0 5)。对照组P水平与MMP 9mRNA表达呈正相关 (r=0 .72 2 ,P <0 .0 1 ) ,而研究组无相关性 (P >0 .0 5) ;两组P水平与TIMP 1mRNA ,E2 与MMP 9、TIMP 1mRNA均无相关性 (P >0 .0 5)。结论 原因不明不孕患者黄体中期P水平影响MMP 9、TIMP 1mRNA的表达并致活性下降 。
Objective To investigate the expression of matrix metalloproteinases 9 (MMP 9) and tissue inhibitor of metalloproteinases 1(TIMP 1) mRNA in the endometrium during mid luteal phase in women with unexplained infertility and its steroidal regulation Methods In situ hybridization method was utilized to detect the location of MMP 9 and TIMP 1 mRNA. Reverse transcription polymerase chain reaction (RT PCR) method was utilized to detect and quantitate MMP 9 and TIMP 1 mRNA expression levels in the endometrium during mid luteal phase. Serum concentration of estradiol (E 2) and progesterone (P) were measured by radioimmunoassay. Thirty eight patients with unexplained infertility were included as study group, and 20 women with male factor infertility or normal volunteers were selected as control group. Results MMP 9 and TIMP 1 mRNA were diffusely distributed in the cytoplasm and neuclei of glandular and stromal cells. Their staining in control group were more abundant than those in study group. Endometrial MMP 9 and TIMP 1 mRNA expression during mid luteal phase showed by RT PCR in the study group were significantly lower than those in the control group (0.42±0.19 Vs 0.57±0.19, P <0.05;0.59±0.19 Vs 0.81±0.20, P <0.01 respectively). Serum P of the study group was significantly lower than that of control group [(34±15) nmol/L Vs (53±17) nmol/L, P <0.01]. Serum P level was positively correlated with MMP 9 mRNA only in control groups Conclusions MMP 9 and TIMP 1 mRNA might play an important role in the process of implantation . The decreased expression of their gene related to P level might be one of the major causes in unexplained infertility.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2003年第6期346-349,i001,共5页
Chinese Journal of Obstetrics and Gynecology
