摘要
目的分析实验性心肌缺血大鼠血浆与心肌8-异前列腺素F2α(8-iso-PGF2α)水平相关性,以及抗氧化剂N-乙酰半胱氨酸(NAC)的治疗效果,探讨血浆8-iso-PGF2α反映心肌氧自由基损伤程度和干预效果的可能性。方法45只Wistar大鼠分为三组:缺血组、缺血+NAC组和对照组。使用垂体后叶素建立大鼠急性心肌缺血模型,以心电图上ST段的抬高作为心肌缺血指标。在缺血+NAC组,预先用NAC 0.1 g/kg·d灌胃3周。应用ELISA方法测定心肌缺血及正常大鼠血浆及心肌组织8-iso-PGF2α含量。结果(1)急性心肌缺血组大鼠的血浆和心肌组织8-iso-PGF2α含量分别为(187.1±45.8)pg/ml和(259.3±47.5)pg/g,明显高于正常对照组的(60.4±13.7)pg/ml和(88.6±16.9)pg/g(P<0.01);(2)急性心肌缺血前应用NAC组的血浆和心肌组织8-iso-PGF2α含量分别为(88.2±16.4)pg/ml和(109.4±24.7)pg/g,明显低于缺血组(P<0.01);(3)血浆与心肌8-iso-PGF2α水平间存在正相关(r=0.856,P<0.01);(4)与正常组比较,缺血组的心电图ST段明显抬高[心肌缺血45 min时抬高最为明显,达(0.34±0.05)mV](P<0.05),心肌缺血前使用NAC,可使心肌缺血得以改善[心肌缺血45 min时心电图ST段仅达(0.18±0.05)mV]。结论急性心肌缺血时8-iso-PGF2α含量产生增加;
Objective To examine the correlation between 8-iso-prostaglandin F 2α (8-iso-PGF 2α ) levels in the lasma and myocardial tissue of rats with myocardial ischemia and observe the intervention effect of N-acetlycysteine (NAC), for the purpose of assessing the value of 8-iso-PGF 2α in estimating the extent of free radical damage and implementing possible interventions. Methods Forty-five Wistar rats were divided into ischemia, ischemia+NAC and control groups, and in the former 2 groups, acute myocardial ischemia models were produced by pituitrin. Elevated ST segment in ECG served as the indicator for myocardial ischemia. Rats in ischemia+NAC group were pre-treated with NAC (0.1 g/kg·d) for three weeks before the ischemia 8-iso-PGF 2α levels in the plasma and myocardial tissue were determined by enzyme-linked immunosorbent assay (ELISA). Results In ischemia group, the 8-iso-PGF 2α levels in the plasma and myocardial tissue were 187.4±45.8 pg/ml and 259.3±47.5 pg/g, respectively, higher than those in the control group (60.4±13.7 pg/ml and 88.6±16.9 pg/g, respectively, P<0.01) and those in ischemia+NAC group (88.2±16.4 pg/ml and 109.4±24.7 pg/g, respectively, P<0.01). A positive correlation was noted between the 8-iso-PGF 2α levels in the plasma and myocardial tissue (r=0.865, P<0.01). In comparison with the control group, elevation of the ST segment of ECG in rats with myocardial ischemia was obvious, and the peak elevation occurred 45 min after ischemia (0.34±0.05 mV, P<0.01). Pre-treatment with NAC proved to help alleviate the subse- quent ischemia, with ST segment elevation of only 0.18±0.05 mV. Conclusions In condition of acute myocardial ischemia in rats, 8-iso-PGF 2α levels tend to increase, which can be indicative of the degree of myocardial ischemia. NAC pre-treatment can alleviate the ischemic condition by offsetting the damage caused by the free radicals.
出处
《第一军医大学学报》
CAS
CSCD
北大核心
2003年第6期605-607,共3页
Journal of First Military Medical University
