摘要
用AFLP分子标记技术对 5 0个有代表性的玉米及其野生近缘种大刍草、摩擦禾、薏苡材料进行了遗传关系分析。利用 2 1个PstⅠ MseⅠ引物组合检出可辨认带共 6 4 1条 ,其中多态性带 5 86条 ,平均每对引物得到 30 5 2条多态性带 ,多态性 91 4 %。聚类分析结果表明 ,可以将 5 0个材料分为 3大类 ,与已有的玉米族分类一致 ,但与玉蜀黍属内的种间和种内分类存在差异 ,说明大刍草各种内存在较大的遗传多样性。本实验发现玉蜀黍属与摩擦禾关系比与薏苡的关系更近 ,但并没有发现玉米与小颖玉米亚种比与其他大刍草的遗传关系更近。
AFLP marker technique was used to investigate the genetic relationships between 50 accessions of maize and its wild relatives including teosinte, Tripsacum and Coix With twenty one AFLP primer combinations of PstⅠ/MseⅠ , 641 AFLP amplified bands were obtained, including 586 polymorphic bands with an average of 30 52 per primer combination and the polymorphism percentage of 91 4% Cluster analysis using UPGMA method based on the data of AFLP showed that the 50 materials could be divided into three groups ( Tripsacum, Zea and Coix ), which agreed with the former taxonomical classification But the groupings under Zea was not consistent with the taxonomy The present study confirmed that maize had closer relationships with Tripsacum than with Coix , but did not find the evidence to support the closer relationships between maize and Zea mays ssp parviglumis , the presumed progenitor of cultivated maize
出处
《作物学报》
CAS
CSCD
北大核心
2004年第4期354-359,共6页
Acta Agronomica Sinica
基金
国家转基因专项 (J0 0 C 0 0 2 0 1)
国家攻关项目 ( 2 0 0 1BA5 11B0 4)资助
