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应用表达谱芯片技术筛选NS5ATP9反式调节基因的研究 被引量：2

Screening of genes trans-regulated by NS5ATP9 with cDNA microarray assay
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摘要 目的:应用基因表达谱芯片技术研究丙型肝炎病毒非结构蛋白5A反式激活蛋白9(NS5ATP9)的反式调节基因.方法:构建NS5ATP9基因的真核表达载体pcDNA3.1(-)-NS5ATP9,应用基因表达谱芯片技术对pcDNA3.1(-)-NSSATP9转染的HepG2(人肝母细胞瘤细胞系)细胞和转染空载体pcDNA3.1(-)的相同细胞的差异表达mRNA进行检测和分析.结果:HepG2细胞经转染NS5ATP9后,有16条差异基因表达水平发生显著改变,其中3条基因表达增强,13条基因表达降低.这些差异表达的基因与细胞的增生、分化、凋亡及细胞的信号转导密切相关.结论:应用基因表达谱芯片成功筛选了丙型肝炎病毒NS5ATP9作用HepG2细胞后差异表达基因,为阐明NS5ATP9的作用提供了新的依据. AIM: To screen the genes trans-regulated by NS5ATP9 with cDNA microarray assay. METHODS: The recombined expressive plasmid pcDNA 3.1(-)-NS5ATP9 was constructed, and HepG2 cells were transfected. Total mRNA was isolated from the HepGZ cells transfected with pcDNA3.1(-) and pcDNA3.1(-)-NS5ATP9, respectively. cDNA microarray was employed for detecting and analyzing of mRNA from both HepG2 cells transfected. RESULTS: From the microarray assay, 3 genes were found up-regulated, and 13 genes down-regulated. Their encoding proteins were involved in cell signal transduction, cell proliferation, cell apoptosis and differentiation. CONCLUSION: cDNA microarray technology is successfully used to screen diversity genes expressed by NS5ATP9 in HepG2 cells, which brings some new clues for the study of the function of NS5ATP9.
作者 李强 梁耀东 成军 王琳 王建军 张健 刘妍 程明亮
机构地区 中国人民解放军第 贵阳医学院传染科
出处 《世界华人消化杂志》 CAS 2004年第2期323-326,共4页 World Chinese Journal of Digestology
基金 国家自然科学基金攻关项目 No.C03011402 No.C30070689军队"九 五"科技攻关项目 No.98D063军队回国留学人员启动基金项目 No.98H038军队"十 五"科技攻关青年基金项目 No.01Q138军队"十 五"科技攻关面上项目 No.01MB135~~
关键词 表达谱芯片技术 NS5ATP9反式调节基因 肝炎病毒 HCV 基因功能 丙型肝炎
分类号 R373.21 [医药卫生—病原生物学]
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参考文献30

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同被引文献33

  • 1成军,杨倩,刘妍,王建军,纪冬.小鼠和大鼠NS5ATP4同源基因序列的生物信息学分析[J].世界华人消化杂志,2004,12(7):1582-1587. 被引量：4
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  • 5Wang Q, Wang Y, Li Y, et al. NS5ATP9 Contributes to inhibition of cell proliferation by hepatitis C virus (HCV) nonstructural protein 5A (NS5A) via MEK/extracellular signal regulated kinase (ERK) pathway[J]. Int J Mol Sci,2013,14(5): 10539-10551.
  • 6Su X, Zhang T, Cheng P, et al. KIAA0101 mRNA overexpression in peripheral blood mononuclear cells acts as predictive marker for hepatic cancer[J]. Tumour Biol,2014,35(3):2681-2686.
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世界华人消化杂志
2004年 第2期

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