摘要
目的 筛选膦甲酸钠处理人T淋巴细胞系Jurkat细胞后的差异表达基因。方法 应用基因表达谱芯片技术对膦甲酸钠处理的Jurkat细胞和以生理盐水处理的相同细胞的mRNA进行检测。结果 Jurkat细胞经膦甲酸钠处理后 ,所检测的 115 2条目的基因中 ,有 94条产生差异表达 ,其中 38条基因表达增强 ,5 6条基因表达降低。结论 应用基因表达谱芯片成功筛选了膦甲酸钠处理淋巴细胞后差异表达基因 。
Objective To screen the genes differentially expressed in gene expression in human lymphoma cell line Jurkat cells treated with phosphonoformate (PFA). Methods cDNA microarray technique was employed to detect the mRNA expressed in Jurkat cells treated with PFA or 0.9 percent sodium chloride, respectively. Results Among 1 152 genes there were 94 genes with different expression, of which 38 genes were upregulated and 56 genes were downregulated in Jurkat cells treated with PFA compared with those treated with 0.9 percent sodium chloride. Conclusions cDNA microarray technique was successfully used to screen the genes with different expression in Jurkat cells treated with PFA, and the results brought some new clues for the study of the immune regulation mechanism of PFA.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2004年第5期386-388,共3页
Medical Journal of Chinese People's Liberation Army
基金
军队回国留学人员启动基金 (编号 98H0 38)
国家自然科学基金攻关项目 (编号C0 30 1 1 4 0 2 0
C30 0 70 689)
军队"十五"科技攻关青年基金项目 (编号 0 1Q1 38)
军队"十五"科技攻关面上项目 (编号 0 1B1 35)资助课题
