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3种富集胃癌患者外周血中胃癌细胞方法的比较 被引量：1

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摘要 目的:建立一种富集胃癌患者外周血中癌细胞的有效方法.方法:先将不同浓度胃癌MGC-803细胞加入到健康人外周血中,再用淋巴细胞分离液收集单核细胞,然后等分成3份,并用3种方法处理-A方法,用CD45磁珠去除白细胞;B方法,用Ber-EP4磁珠富集癌细胞;C方法,先后使用上述2磁珠.最后用定量RT-PCR检测看家基因β2微球蛋白(B2MG)mRNA的量.同法检测30例胃癌患者外周血.结果:β2MGmRNA扩增曲线的交叉点(crossingpoint,Cp)数值与癌细胞数间有良好的负相关(方法A,P<0.05,方法B或C,P<0.01).方法C检测到的Cp值为3种方法中最大的;其灵敏度为1mL血中含有1个癌细胞.用A、B和C方法检测30例胃癌患者外周血癌细胞的阳性率分别为70.00%,60.00%和36.57%(C与A比较,x2=5.79,P<0.05;C与B比较,x2=4.92,P<0.05).结论:在检测胃癌患者外周血癌细胞的实验中,联用阴性和阳性免疫磁珠可减少来自外周血白细胞的污染.
作者 陈健 郭俊明 金之瑾 肖丙秀
机构地区 宁波大学医学院
出处 《世界华人消化杂志》 CAS 2004年第2期461-464,共4页 World Chinese Journal of Digestology
基金 浙江省医药卫生科研基金资助项目 No.2002A073宁波市重点博士基金资助项目 No.02J20101-07宁波大学重点科研项目 No.z0214015
关键词 外周血 胃癌细胞 肿瘤 淋巴细胞
分类号 R735.2 [医药卫生—肿瘤]
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参考文献20

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二级参考文献28

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世界华人消化杂志
2004年 第2期

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