摘要
目的:从水液代谢来研究慢性浅表性胃炎脾胃湿热证与AQP4表达的关系.方法:慢性浅表性胃炎患者32例,其中脾胃湿热证20例.脾气虚证12例;另10例正常人为对照.胃镜下取胃体上部黏膜,观察黏膜炎症情况,免疫组化法、图像分析系统半定量AQP4的蛋白表达量.结果:脾胃湿热证胃黏膜的炎症明显要重于脾虚证和正常人组(中重度比17/20vs6/12,0/10,P<0.05,P<0.01);脾胃湿热证AQP4蛋白表达量强于脾虚证组(209±59vs127±61,P<0.01)和正常人组(vs164±32,P<0.05);脾虚证蛋白表达量低于正常人组,但两组无显著性差异(127±61vs164±32,P>0.05).结论:AQP与水液代谢密切相关,AQP的异常表达可能是脾胃湿热证的发生机制之一.
AIM: To study the relationship between piwei damp-heat syndrome and aquaporin 4 (AQP4) from fluid metabolism in patients with chronic superficial gastritis. METHODS: A total of 32 patients with chronic superficial gastritis were composed of 20 cases of piwei damp-heat syndrome and 12 of pi-qi deficiency syndrome, while 10 cases of healthy people were taken as control. The mucosa of upper stomach was obtained by gastroscopic biopsy. The mucosal inflammation was observed and the protein expression of AQP4 was semiquantitated by the immuno-histochemistry and image assay system. RESULTS: The mucosal inflammation of piwei damp-heat syndrome group was more severe than that of the other two groups significantly (moderate or severe rate 17/20 vs 6/12, 0/10, P<0.05, P<0.01, respectively), and the protein expression of AQP4 in piwei damp-heat syndrome group was also higher than that of the other two groups significantly (209±59 vs 127±61 and 164±32, P<0.01, P<0.05, respectively). CONCLUSION: AQP has a close connection with water metabolism. The abnormal expression of AQP may be one of the pathogenesis of piwei damp-heat syndrome.
出处
《世界华人消化杂志》
CAS
2004年第2期379-381,共3页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30271572~~
