摘要
目的:通过比较线粒体基因组(mitochondrialDNA,mtDNA)拷贝数在胃癌和癌旁胃黏膜组织间的差异,阐述mtDNA与胃癌发生的关系.方法:PCR分别扩增胃癌组织和癌旁胃黏膜组织各20例共40个样本的线粒体D-loop两个高变区HV1(hypervariableregion)和HV2;并以核基因组的β-actin作为定量标准物.聚丙烯酰胺凝胶电泳(polyacrylamidegelelectrophoresis,PAGE)银染比较mtDNA拷贝数在癌和正常组织间的差异.结果:HV1和HV2拷贝量(用β-actin标准化)在胃癌组织和癌旁组织间有显著的差异(P<0.01);其拷贝量与组织类型,癌组织浸润深度未发现有统计学联系(P>0.05);而与核内一些重要的酶:碱性磷酸酶(AKP)、环腺苷酸磷酸二脂酶(cAMP-PDE)和环鸟苷酸磷酸二脂酶(cGMP-PDE)表达有一定关系(P<0.05).结论:胃癌的发生与胃上皮细胞内mtDNA量的减少有着密切的关系.有望成为一种新的肿瘤分子标志物.
AIM: To explore the relationship between mitochondrial DNA (mtDNA) and gastric cancer by comparing the difference of mtDNA copy number in gastric cancers and paracancerous tissues. METHODS: Hypervariable reigon (HV)1 and HV2 of mitochondrial D-loop region from 20 cases of gastric cancer and 20 paracancerous tissues were amplified by PCR; meantime β-actin was served as a quantitative standard marker, followed by polyacrylamide gel electrophoresis (PAGE) and silver staining, in which the difference of mtDNA copy number was compared between gastric cancers and paracancerous tissues. RESULTS: There existed significantly quantitative difference in HV1, HV2 (standardized with β-actin) between gastric cancers and paracancerous tissues (P<0.01). mtDNA copy number was associated with important enzymes in nucleus such as AKP, cAMP-PDE and cGMP-PDE (P<0.05), although not with tumor histological type and invasive depth (P>0.05). CONCLUSION: The occurrence of gastric cancer is closely associated with decreased mtDNA copy number, which may be a new tumor marker.
出处
《世界华人消化杂志》
CAS
2004年第2期258-261,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30371607~~
