摘要
目的:研究COX-2在胃癌中的表达及其与血管生成的关系,探讨其在胃癌转移中的作用及与胃癌病理生物学行为的关系．方法:采EnVision方法检测胃癌组织芯片中 COX-2的表达,用CD34进行微血管内皮细胞染色,计算微血管密度(MVD),分析其相关性．结果:COX-2在胃癌中的表达明显高于正常胃黏膜(P=0．001)．COX-2的高表达与胃癌的转移(P=0．019)和胃壁浸润深度(0．031)呈正相关,与胃癌的组织病理分型无关(P=0．495), 与Borrmann分型无关(P=0．109)组织MVD (65．49±20．64)明显高于正常胃黏膜组织 (36．21±18．47,P=0．001)．MVD值与胃癌的组织病理分型(P=0．003)和转移有关(P=0．043), 与胃癌胃壁浸润深度(P=0．627)和Borrmann 分型(P=0．634)无明显相关性．COX-2表达阳性组的MVD指数明显高于COX-2表达阴性组 (68．59±19．8 vs 25．82±7．76．P<0．05),COX-2 表达与MVD呈正相关(P=0．001)．结论:组织芯片技术对于快速检测胃癌及其他肿瘤的分子病理学改变是一个强有力的工具．COX-2表达可能通过促进血管形成对胃癌的发生、发展起重要作用,其可作为判断预后和指导治疗的有效指标．
AIM： To explore the expression and clinicalpathological significance of cyclooxygenase-2 （COX-2） as well as its relations with the angiogenesis in the carcinogenesis and metastasis of gastric cancer. METHODS： The expression of COX-2 was detected by EnVisiorr immunohistochemical method in tissue microarray prepared from gastric cancer patients. The microvessel density （MVD） was calculated after CD34 staining for microvessel endothelium. Their correlations were analyzed. RESULTS： The expression of COX-2 in gastric cancer was significantly higher than that in normal controls （P = 0.001）. The over-expression of COX-2 in gastric cancer was positively related to the metastasis and the depth of invasion （P = 0.031）, while it was not related with the histological types （P = 0.495） and Borrmann types （P = 0.328）. The MVD in gastric cancer tissues （65.49 ± 20.64） was notably higher than that in normal controls （36.21 ± 18.47, P = 0.001）. MVD was correlated with the histological types （P = 0.003） and metastasis （P = 0.043）, while it was not correlated with the depth of invasion （P =0.627） and Borrmann types （P = 0.634）. The MVD of COX-2 positive group was higher than that of COX-2 negative one （68.59 ± 19.8 vs 25.82±7.76, P 〈 0.05）, and the expression of COX-2 and MVD were positively correlated （P = 0.001）. CONCLUSION： Tissue microarray is a powerful tool for rapid identification of the molecular alterations in gastric cancer and other pathological types. The expression of COX-2 may play an important role in gastric carcinogenesis by promotion of angiogenesis. It may serve as a marker in the prognosis of gastric cancer.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第6期550-556,共7页
World Chinese Journal of Digestology
基金
国家自然基金资助项目
No.30371607~~
