摘要
目的 探讨尿胰蛋白酶抑制剂 (Urinarytrypsininhibitor ,UTI)缓解重症急性胰腺炎 (Severeacutepancreatitis ,SAP)病变的作用机制。方法 应用柱层析法分离纯化得到高纯度UTI;在大鼠胰腺实质内注射 5 %牛磺胆酸钠液建立大鼠急性出血坏死型胰腺炎模型 ;酶联免疫吸附法动态检测大鼠正常对照组、SAP模型组和UTI治疗组各组IL 6水平。结果 ①获得了高纯度的UTI ;②成功制备大鼠SAP模型 ;③SAP组大鼠IL 6较NC组显著升高 ,给与UTI治疗显著降低。结论 UTI减轻大鼠SAP模型的病理损伤程度 ,与UTI抑制IL 6表达有关。
Objective To explore the role of human urinary trypsin inhibitor(UTI) in severe acute pancreatitis (SAP). Methods UTI was highly purified from the urine of patients with acute pancreatitis by column chromatography. SAP models were established by injection of 5% sodium taurocholate(NaTc) at dose of 0.1 ml/100 g weight under pancreatic capsule. The interleukin 6(IL 6) level in each group was measured by ELISA. Results ① The highly purified UTI with high yield was harvested. ② SAP models were established successfully. ③ The level of IL 6 in SAP group was significantly higher than that in NC, but in UTI group, the increase was not obvious. Conclusion The alleviating effect of UTI on the pathological damage degree in rats with SAP is related to the inhibitive effect of UTI on the expression of IL 6.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第12期1076-1078,共3页
Journal of Third Military Medical University
