摘要
目的 探讨用消化培养法体外培养牛眼小梁细胞,为进一步研究原发性开角型青光眼(POAG)提供实验材料。方法 0.25％胰蛋白酶、0.3％胶原酶等量混合行牛眼小梁细胞的原代培养和传代实验。结果 成功培养出性状良好的牛眼小梁细胞。结论 消化法培养牛眼小梁细胞可获得生长形态及特征稳定的小梁细胞,是值得提倡的培养方法。
Objective To search the culture method of bovin trabecular cells in vitro to provide the necessary condition for experimental study of primary open angle glacoma( POAG) . Methods 0.25% pencreatin and 0. 3% collagenase were equally mixed to get the primary bovine trabecular meshwork cell, and only 0. 25% pencreatin for passage of culture Results Stable growth status of bovine trabecular meshwork cells were successful cultured by digestion method in vitro. Conclusions Digestion method is operative for primary and passage culture of trabecular meshwork cells.
出处
《眼科研究》
CSCD
北大核心
2003年第4期406-408,共3页
Chinese Ophthalmic Research
基金
陕西省基金资助(2000SM29)
关键词
消化法
牛眼
小梁细胞
体外培养
trabecular meshwork cell digestion method culture
