摘要
目的探索体外培养人眼小梁细胞的方法，为进一步研究原发性开角型青光眼的发病机理提供实验的依据。方法用器官培养及组织块培养两种方法，进行人眼小梁细胞的原代培养和传代实验，并同时对照培养人眼巩膜成纤维细胞，对二者从形态学上进行比较。结果（１）器官培养较单纯组织块培养更易获得原代小梁细胞；（２）传３～５代人眼小梁细胞处于最稳定阶段，可利用此阶段细胞进行多种体外实验。结论体外培养人眼小梁细胞可获得生长形态及特征稳定的小梁细胞。
Objective To search the method of hunan （rabecular cell） （HTC） cultured in vitro, which could provide the necessary condition of experimental study on primary open angle glaucoma. Method Organ and tissue culture were taken to get the different passages of human trabecular phology. Results （1） It took rather shorter time to get HTC by using organ culture method than simple tissue culture. （2）The 3rd to 5th passage of HTC was the most stable growth period, at that time they could be used in experiment Conclusion Stable growth shape and characteristic HTC could be obtained by HTC culture in vitro.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
1998年第2期121-123,共3页
Chinese Journal of Ophthalmology
