摘要
For the determination of vitamin B 6 derivatives, a high performance liquid chromatography(HPLC) system composed of a single reversed-phase octadecylsilane column and fluorescence detector with an isocratic elution was proposed. This HPLC system perfectly analyzed pyridoxine, pyridoxal, pyridoxamine, pyridoxine 5′-phosphate and pyridoxamine 5′-phosphate together with pyridoxic acid in biological samples. In this method, relatively low fluorescence intensity of pyridoxal 5′-phasphate was improved by converting it to pyridoxic 5′-phosphate with KCN. The analytical procedure is very simple and economical, it is a useful tool for studying vitamin B 6 in animal body.
For the determination of vitamin B 6 derivatives, a high performance liquid chromatography(HPLC) system composed of a single reversed-phase octadecylsilane column and fluorescence detector with an isocratic elution was proposed. This HPLC system perfectly analyzed pyridoxine, pyridoxal, pyridoxamine, pyridoxine 5′-phosphate and pyridoxamine 5′-phosphate together with pyridoxic acid in biological samples. In this method, relatively low fluorescence intensity of pyridoxal 5′-phasphate was improved by converting it to pyridoxic 5′-phosphate with KCN. The analytical procedure is very simple and economical, it is a useful tool for studying vitamin B 6 in animal body.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2004年第4期638-640,共3页
Chemical Journal of Chinese Universities
基金
教育部留学回国人员科学研究启动基金 ( 2 0 0 2年 )资助
关键词
生物体
维生素B6
高效液相色谱技术
定量分析
High performance liquid chromatography(HPLC)
KCN treatment
Fluorescence detection
Vitamin B 6
