摘要
经肾活检和临床资料确诊的LN患者 5 3例 ,采用免疫荧光双标记染色与低照度荧光图像分析检测方法 ,观察到LN患者肾组织中树突状细胞分布的面积、数量及密度均明显增多。树突状细胞主要分布于肾小管间质和肾血管 ,而肾小球基本未见分布。此外 ,树突状细胞在肾小管间质分布程度与肾小管间质病变程度及肾功能呈正相关。提示树突状细胞可能参与了狼疮性肾炎的发病机制 。
To evaluate the localization of dendritic cells (DC) in kidneys with lupus nephritis (LN) and its pathological significance, biopsy specimen of 53 patients with lupus nephritis and 10 normal individuals were investigated by double immunostaining method and the images were analyzed with Axioplan 2 microscopy. There were much more DC in LN kidneys than in normal kidneys. In renal tissues of patients with LN, DC were found in renal tubules, interstitium and vessels but glomeruli. The distribution area, number and density of DC in renal tissues of severe tubulointerstitial lesions group was greater than mild and moderate group. This study preveals that mature DC are recruited into the kidney with nephritis which is associated with tubulointerstitial lesions, suggesting DC plays an important role in pathogenesis of lupus nephritis.
出处
《现代免疫学》
CAS
CSCD
北大核心
2004年第2期142-145,共4页
Current Immunology
基金
国家自然科学基金资助项目 ( 3 9970 3 40 )
上海市自然科学基金资助项目 ( 0 2ZB14 0 41
0 3 4119916)
