摘要
AIM:To study whether vascular endothelial growth factor messenger RNA (VEGF mRNA) in the hepatocellular carcinoma (HCC) tissues obtained after curative resection has a prognostic value.METHODS: Using a reverse-transcription polymerase chain reaction (RT-PCR)-based assay, VEGF mRNA was determined prospectively in liver tissues of 50 controls and in HCC tissues of 50 consecutive patients undergoing curative resection for HCC.RESULTS: Among the isoforms of VEGF mRNA, VEGF165 and VEGF121 were expressed. By multivariate analysis, a higher level of VEGF165 in HCC tissue correlated with a significant risk of HCC recurrence (P=0.038) and significantly with recurrencerelated mortality (P=0.045); while VEGF121 did not. Other significant predictors of HCC recurrence included cellular dedifferentiation (P=0.033), an absent or incomplete capsule(P=0.020), vascular permeation (P=0.018), and daughter nodules (P=0.006). The other significant variables of recurrence related mortality consisted of vascular permeation (P=0.045),and cellular dedifferentiation (P=0.053). The level of VEGF mRNA in HCC tissues, however, did not significantly correlate with tumor size, cellular differentiation, capsule, daughter nodules,vascular permeation, necrosis and hemorrhage of tumors.CONCLUSION: The expression of VEGF mRNA, especially isoform VEGF165,in HCC tissues, may play a significant and independant role in the prediction of postoperative recurrence of HCC.
AIM:To study whether vascular endothelial growth factor messenger RNA (VEGF mRNA) in the hepatocellular carcinoma (HCC) tissues obtained after curative resection has a prognostic value. METHODS:Using a reverse-transcription polymerase chain reaction (RT-PCR)-based assay,VFGF mRNA was determined prospectively in liver tissues of 50 controls and in HCC tissues of 50 consecutive patients undergoing curative resection for HCC. RESULTS:Among the isoforms of VEGF mRNA,VEGF_(165) and VEGF_(121) were expressed.By multivariate analysis,a higher level of VEGF_(165) in HCC tissue correlated with a significant risk of HCC recurrence (P=0.038) and significantly with recurrence- related mortality (P=0.045);while VEGF_(121) did not.Other significant predictors of HCC recurrence included cellular dedifferentiation (P=0.033),an absent or incomplete capsule (P=0.020),vascular permeation (P=0.018),and daughter nodules (P=0.006).The other significant variables of recurrence related mortality consisted of vascular permeation (P=0.045), and cellular dedifferentiation (P=0.053).The level of VEGF mRNA in HCC tissues,however,did not significantly correlate with tumor size,cellular differentiation,capsule,daughter nodules, vascular permeation,necrosis and hemorrhage of tumors. CONCLUSION:The expression of VEGF mRNA,especially isoform VEGF_(165),in HCC tissues,may play a significant and independant role in the prediction of postoperative recurrence of HCC.
