摘要
AIM:To examine the expression of nuclear factor kappaB(NF-κB)and its target genes in intestinal metaplasia(IM),dysplasia(DYS)and gastric carcinoma(GC)infected with Helicobacter pylori(H pylori)and to investigate the mechanism underlying Hpyloricytotoxin associated gene A(cag A)infection leading to gastric adenocarcinoma.METHODS:Expressions of NF-κB/p65 and its target genes:c-myc,cyclinD1 and bcl-xl were immunohistochemically examined in 289 cases of gastric biopsy and resection specimens from patients with IM,DYS and GC infected with H pylori.H pylori in the above mentioned tissues was detected by Warthin-Starry stain and rapid urease tests.IgG antibody to cagA in sera of the patients was measured by ELISA.RESULTS:The positive rates of NF-κB/p65 were significantly higher in groups with cagA of IMI-Ⅱ(28/33),IM III(48/52),DYSI(27/31),DY5Ⅱ-Ⅲ(28/32),GC(35/40)than in groups without cagA of IMI-Ⅱ(4/17),IMⅢ(3/20),DYSI(3/20),DYSⅡ-Ⅲ(6/21),GC(10/23).The expressions of c-myc,cyclinD1,and bcl-xl were significantly higher in groups with cagA of IMⅢ(47/52,49/52,46/52),DYSⅡ-Ⅲ(29/32,26/32,25/32)than in groups without cagA of IMⅢ(8/20,7/20,5/20),DYSⅡ-Ⅲ(10/21,8/21,3/21),which were in conformity with the expression of NF-κB in IMⅢ,and DYSⅡ-Ⅲ.Asignificantly higher expression level of NF-κB/p65,c-myc,cyclinD1 and bcl-xl was detected in intestinal type GC(27/28,18/28,22/28,24/28)than in diffuse type GC(8/12,3/12,3/12,6/12),respectively.CONCLUSION:There may be two different molecular mechanisms in the occurrence of intestinal and diffuse type gastric carcinomas.Intestinal type gastric carcinoma is strongly associated with high expression of c-myc,cyclinD1 and bcl-xl through NF-κB/p65 activated by Hpylori cagA.Inhibiting the activity of NF-κB is an effective and promising way to prevent intestinal type gastric carcinoma.
基金
Supported by grants from the Bureau of Science and Technology of Hubei Province,No.2003AA301C27
NF-κB
胃癌
癌前病变
幽门螺杆菌
目标基因
细胞因子
