摘要
用 2对稻瘟病菌的两性能育菌株 (交配型 1.1和交配型 1.2 )测定了来自湖南烟溪病圃上的 4年 12 4个稻瘟病菌株的交配型 ,用微卫星分子标记分析其中 10 5个菌株的遗传多样性及其亲缘关系。12 4个菌株中 ,2 8个为交配型 1.1,39个为交配型 1.2 ,5 7个不育 ,分别占供试菌株的 2 2 .5 8%、31.4 5 %和 4 5 .97% ;在 0 .6 7相似性的基础上 ,7对微卫星引物扩增出的标记可将 10 5个菌株区分为 6个系谱群。
One hundred and twenty-four Magnaporthe grisea isolates collected in four years from the Yanxi Blast Nursery, Hunan Province were tested for mating type with four hermaphroditic fertile isolates MAT1.1 (KA3 and TH12) and MAT1 2 (Guy11 and TH16) and the genetic diversity of 105 isolates were analyzed by using microsatellite markers(SSR). Among the 124 isolates, 28 belonged to MAT1.1, 39 to MAT1.2 and the remaining 57 to sterile ones, taking 22.58%, 31.45% and 45 97% for each group, respectively. The 105 isolates could be divided into six lineages at 0.67 similarity level based on the amplicons with seven pairs of SSR primers.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2004年第3期262-268,共7页
Chinese Journal of Rice Science
基金
欧盟项目 (ICA4 CT 2 0 0 0 3 0 0 2 1)
