摘要
应用差异显示技术研究了NaHCO3胁迫下紫杆柽柳(Tamarix androssowii)基因的表达。经Norlhern检测共获得了17个基因片段,BLASTX分析表明,有2个基因与编码F-box类蛋白家族成员的基因同源性较高(F-box蛋白的功能为调节细胞周期转变、转录调控和信号转换,在植物抗逆防御系统中起重要作用);1个基因与翻译起始因子eIF成员有高的同源性(eIF在植物和酵母的抗盐胁迫中起重要作用);2个与分泌型过氧化物酶和过氧化物酶基因同源性高的基因片段;5个与盐碱胁迫密切相关的新基因,它们在胁迫前后差异表达明显。另外7个与已知基因同源性较高或有一定相似性,它们在抗盐碱胁迫中的作用尚待研究。
Differential display technique was used to study the gene expression of Tamarix androssowii treated with NaHCO3 solution. Seventeen differentially expressed genes were obtained through Northern detection (Figs. 1-3). The result of BLASTX analysis shows that two of these genes (GenBank accession number CD028574 and CD028579) are homologous to the F-box protein family member, which can regulate diverse cellular processes, including cell cycle transition, transcription regulation and signal transduction, by playing roles in Skplp-cullin-F-box protein (SCF) complexes or non-SCF complexes. F-box protein plays a very important role in plant defence system. One gene (GenBank accession number CD028589) is highly homologous to the translation initiation factor eIF, which regulates the proteins translation and is an important determinant of sodium tolerance in yeast and plants. Additionally, two genes (GenBank accession numbers CD028584 and CD028586) that are homologous to secretory peroxidase and peroxidases were identified, peroxidases are familiar proteins expressed in plant under stress. Five new genes closely related to salt and alkali resistance were obtained, which were expressed quite differentially when treated by NaHCO3 solution (Figs. 1,2). Seven differentially expressed genes were obtained which have highly or some homologous to the known genes, their function in stress need to be further studied. These seventeen genes were accepted as novel cDNA sequences by GenBank and were given them new accession numbers, their accession numbers were CD028574-CD028581 and CD028583-CD028591.
出处
《植物生理与分子生物学学报》
CAS
CSCD
2004年第2期229-233,共5页
Journal Of Plant Physiology and Molecular Biology
基金
国家"973"计划项目(No.G1 999016000)资助~~
