摘要
目的 研究32 P放射性同位素液体球囊防治血管损伤后再狭窄的可行性 ,并探讨p2 7、增殖细胞核抗原 (PCNA)在血管内放射治疗过程中的变化。方法 4 0只雄性大耳白兔随机分为放射治疗组和对照组 ,行单侧髂动脉球囊过度扩张损伤术 ,放射治疗组在球囊内灌注液体放射线同位素 32 P ,给予 2 0Gy照射 ,对照组充盈假源对照。分别于第3、2 8天处死动物 ,取髂动脉 ,行免疫组织化学染色 ,检测p2 7,PCNA阳性细胞的表达 ,应用计算机图像分析系统测定新生内膜面积。结果 第 3天时放射治疗组p2 7阳性细胞百分率明显高于对照组 ,第 2 8天时放射治疗组新生内膜面积明显低于对照组 ,而p2 7的阳性细胞百分率仍高于对照组。放射治疗组PCNA阳性细胞百分率第 3、2 8天明显低于对照组。实验未见动脉瘤、坏死等不良病理改变。结论 32 P放射性同位素液体球囊抑制血管损伤后的内膜增生有良好的可行性和安全性。血管内放射治疗显著抑制PCNA的表达 ,p2 7可能在放射治疗抑制再狭窄的过程中起重要作用。
Objective To evaluate the efficacy of irradiation with 32 P radioisotope liquid filled balloon in preventing the restenosis after artery injury and to investigate the changes of p27 and proliferating cell nuclear antigen (PCNA) during vascular radiotherapy.Methods Forty male rabbits were randomized into radiotherapy group and control group.The iliac artery injury model was established by balloon over-stretching.In radiotherapy group,the balloon filled with 32 P radioisotope liquid was placed at the injured segment,while in control group,the balloon was filled with water.The animals were sacrificed on days 3 and 28 after the artery injury. The expression of PCNA and p27 was estimated using immunohistochemistry. Neointimal area was measured by computer analysis of photomicrograms.Results Expression of p27 was significantly higher in radiotherapy group than in control group on days 3 and 28.Radiotherapy decreased significantly expression of PCNA in radiotherapy group as compared with control group on days 3 and 28.Radiotherapy significantly reduced the neointimal area in radiation group on day 28.Conclusions These results indicate that the use of liquid 32 P is safe and feasible to prevent restenosis.Radiotherapy decreases significantly expression of PCNA.p27 may play an important role in preventing the restenosis by the vascular radiotherapy.
出处
《中华老年心脑血管病杂志》
CAS
2004年第2期116-118,共3页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
