摘要
目的 探讨人参皂甙对巨噬细胞表达造血生长因子的影响。 方法 采用造血祖细胞体外培养、造血生长因子生物学活性检测、免疫细胞化学与核酸原位杂交等技术 ,研究人参总皂甙 (TSPG)对大鼠骨髓巨噬细胞(rBMM)和人单核细胞株 (THP 1)表达造血生长因子的影响。 结果 经TSPG(10～ 5 0mg L)诱导制备的rBMM和THP 1的培养上清液可显著提高大鼠和人的CFU Mix、CFU GM和CFU E的集落产率 ;经TSPG诱导后rBMM和THP 1表达EPO、GM CSF、IL 3、IL 6的蛋白水平有不同程度提高 ;经TSPG诱导后rBMM和THP 1表达EPO、GM CSF和IL 3的mRNA水平和强度显著提高。 结论 TSPG可通过直接和 或间接途径刺激造血诱导微环境的巨噬细胞 ,从蛋白水平和基因转录水平两级层次上促进造血调控因子 (如EPO、GM CSF、IL 3和IL 6等 )的合成和分泌 ,进而促进造血干 祖细胞的增殖分化 ,这或许是人参调节血细胞生成的可能途径之一。
Objective To investigate the effect of saponins of Panax Ginseng on expression of hematopoietic growth factors in macrophage and explore the biological mechanism for hematopoiesis regulated by Panax Ginseng. Methods By using the hematopoietic progenitor culture in vitro, biological activity assay of hematopoietic growth factor(HGF), immunocytochemistry and in situ hybridization, the HGF-inducing effects of rat bone marrow macrophage(rBMM)and human monocyte line(THP-1) treated with total saponins of Panax Ginseng(TSPG) were studied. Results The results indicated that the conditioned media of rat bone marrow macrophage (rBMM-CM) and human monocyte line(THP-1-CM) prepared with TSPG(10-50mg/L) can increase significantly the formation of CFU-Mix CFU-GM, and CFU-E in mice and human; the expression of Epo, GM-CSF, IL-3 and IL-6 protein in rBMM and THP-1 treated with TSPG(50mg/L) is stronger than that of control group; the expression of EpomRNA, GM-CSF mRNA and IL-3 mRNA in rBMM and THP-1 induced by TSPG(50mg/L) is intensified.Conclusion We speculate that TSPG can induce the macrophage in hematopoietic inductive microevironment to synthesize and secrete HGF, such as Epo, GM-CSF, IL-3 and IL-6 through direct and/or indirect pathway, which can promote the hematopoiesis. This may be one of the cellular and molecular mechanisms of Panax Ginseng regulating hematopoiesis.
出处
《解剖学报》
CAS
CSCD
北大核心
2003年第5期500-505,共6页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目 (396 70 880 )
关键词
人参皂甙
诱导
骨髓巨噬细胞
表达
造血生长因子
Saponins of Panax Ginseng
Macrophage
Hematopoietic progenitor
Hematopoietic growth factor
Rat
