摘要
未刺激和经大肠杆菌脂多糖(LPS)刺激的腹腔巨噬细胞培养上清液对早期红系祖细胞(BFU-E)增殖均呈促进作用,对晚期红系祖细胞(CFU-E)及粒-单核系祖细胞(CFU-GM)增殖呈双向调控,即低浓度有促进作用,高浓度有抑制作用。其中经LPS刺激的腹腔巨噬细胞培养上清液具有更高的调控活性。这种培养液对多能造血干细胞(CFU-S)的增殖似无明显作用。本研究初步探讨了腹腔巨噬细胞培养上清液的造血调控机理,提示腹腔巨噬细胞培养上清液有促红细胞生成素(EPO)、爆式集落刺激活性(BPA)、粒-单核系集落刺激因子(CSF-GM)样活性,LPS可使巨噬细胞培养上清液中EPO、BPA和CSF-GM样活性增强。
BFU-E growth is enhanced significantly after addition of either non-stimulated or LPS-stimulated peritoneal macrophage culture supernatants. This supernatant shows positive and negative control on the growth of CFU-E and CFU-GM, low concentration supernatant shows promoting activity and high concentration supernatant presents inhibition. The LPS-stimulated supernatant shows much stronger promoting activities than non-stimulated supernatant. This supernatant has no obviously action on the proliferation of CFU-S. The mechanism of the regulation of mouse hemopoiesis by the conditioned media of the peritoneal macrophage is discussed. Our results show that this supernatant of cultured peritoneal macrophages may contain EPO-like, BPA-like and CSF-GM-like factors.
出处
《解剖学报》
CAS
CSCD
北大核心
1992年第3期300-305,共6页
Acta Anatomica Sinica
关键词
巨噬细胞
培养
上清液
大肠杆菌
脂多糖
造血调控
小鼠
Macrophage culture supernatant
Lipopolysaccarides
Hemopoiesis regulation
Mouse
