摘要
目的 探讨胃黏膜瘦素、瘦素受体的表达和信号转导及在H .pylori相关性胃炎发生发展过程中的作用。 方法 胃镜直视下取 4 5例就诊前两周内未行抗H .pylori治疗的慢性胃炎患者 (无糖尿病、糖耐量异常 )胃窦部和体部黏膜组织。应用快速尿素酶试验、组织切片Warthin Starry银染和H .pylori UreA PCR技术进行H .pylori诊断。应用ELISA法测定胃黏膜瘦素含量。应用原位杂交和免疫组化技术分别对OB RmRNA和STAT 3蛋白进行测定。结果 ①H .pylori阳性组胃体部瘦素含量明显高于阴性组。②OB RmRNA存在于壁细胞和胃窦基底侧腺细胞。③STAT 3蛋白表达于壁细胞。STAT 3核着色程度与瘦素含量呈明显正相关。H .pylori阳性组核STAT 3含量明显高于阴性组。 结论 瘦素存在于胃黏膜组织内 ,OB RmRNA表达于壁细胞和胃窦部基底侧腺细胞。STAT 3可能是壁细胞瘦素受体的信号分子。H .
Objective To investigate the expression and signaling pathway of leptin andits receptors in gastric mucosa and provide further evidence for the involvement of gastric leptin in the contribution to the occurrence and development of Hpylori-associated gastritis. Methods Under endoscope 45 patients' gastric biopsy specimens were obtained, including the corpus and antrum gastric mucosae. The presence of H.pylori was detected by rapid urease test, Warthin-Starry staining and H.pylori-UreA-PCR. Gastric mucosa leptin content was determined by ELISA. Cellular localization and expression of OB-R mRNA and STAT-3 protein were assessed by in situ hybridization and immunohistochemistry,respectively. Results Leptin content of the corpus gastric mucosa in H.pylori-positive patients was significantly increased [(4.62±0.52) ng/ml, n=15] as compared with the H.pylori-negative group [(2.50±0.40) ng/ml,n=9]. The presence of OB-R mRNA was shown in parietal cells and antral fundicglandular cells, and its distribution and density were similar in two groups. By immunohistochemistry STAT-3 protein was detectable only in parietal cells. The amount of STAT-3 protein immuno-staining positive nucleuses were correlatedto the gastric leptin level, and were significantly higher in H.pylori-positive group than H.pylori-negative group. Conclusions The coexpressionof leptin receptors and STAT-3 are found in parietal cells that argues for STAT-3 as a signal molecule of leptin in human stomach. H.pylori-associated gastritis leads to significant increases in local leptin concentration in the gastric corpus.
出处
《山西医科大学学报》
CAS
2004年第2期122-125,共4页
Journal of Shanxi Medical University
