摘要
开发了固定化假丝酵母脂肪酶 99 12 5合成棕榈酸异辛酯的工艺 .对反应温度、酶用量、底物摩尔比等酯化反应条件进行了研究 .脂肪酶以吸附的形式固定在织物膜上 ,以 1g棕榈酸、 0 6 7g异辛醇、 0 12g固定化脂肪酶和 5ml石油醚组成的反应系统在 4 0℃条件下反应 2 4h ,酯化率可达 96 6 % .固定化酶连续反应 9批后酯化率仍维持在 90
The synthesis of 2-ethylhexyl palmitate by immobilized lipase candida sp. 99-125 was investigated.The lipase was immobilized on fiber membrane by adsorption. The conditions of enzymatic synthesis of 2-ethylhexyl palmitate were optimized. In the reaction system composed of 1 g palmitic acid, 0.67 g 2-ethyl hexanol, 5 ml petroleum ether and 0.12 g (1000 IU) immobilized lipase ,shaken in a 50 ml conical flask at 40 ℃ for 24 h,the esterification rate reached 97%. The reuse stability of immobilized lipase was ab...
基金
国家高技术研究发展计划 (863 )项目(No 2 0 0 2AA5 14 0 3 0 )
国家"十五"攻关项目 (No 2 0 0 1BA70 8B0 3 0 8)
国家自然科学基金项目 (No 2 0 1760 0 2 )
国家重点基础研究发展规划 973 )项目(No 2 0 0 3CB7160 0 2 )
中石化(No 2 0 2 0 5 9)资助~~
