摘要
以大孔吸附树脂为载体,将来源于Candidarugosa的脂肪酶用吸附法固定化后用谷氨酸双十二烷基酯核糖醇进行包衣,用于在有机溶剂中催化月桂酸和月桂醇的酯化反应。结果表明,AB 8型大孔吸附树脂为最佳载体,最适有机溶剂为异辛烷,酶固定化后再包衣,酶的比活较未包衣前提高了60%—90%。
The lipase from Candida rugosa was first immobilized on macroporous adsorption resins, then coated with glutamic acid didodecyl ester ribitol amide as a catalyst for esterification of lauric acid and lauryl alcohol in (organic) solvents. The resin AB-8 was proved to be the best carrier and isooctane was the most suitable solvent.The specific activity of the surfactant-coated immobilized lipase was 1.6 to 1.9 times higher than that of the uncoated immobilized lipase.
出处
《化学工程》
CAS
CSCD
北大核心
2004年第5期50-52,共3页
Chemical Engineering(China)
基金
国家自然科学基金资助项目(29876031)
