摘要
目的 :对口腔癌相关成纤维细胞 (CAFs)的生长增殖特性进行研究。方法 :复苏前期实验中冻存的第3代纯化舌CAFs及同部位的正常成纤维细胞 (NFs) ,在保证培养条件、接种细胞量、观察时间基本一致的情况下 ,比较二者的生长曲线、群体倍增时间、有丝分裂指数曲线 ,并用MTT法测定细胞的增殖活力。结果 :从总体上比较 ,CAFs的生长速度、分裂增殖能力及存活能力均较NFs明显增强 ( p <0 .0 5 )。CAFs和NFs的细胞群体倍增时间分别为 60 .19h和 76.61h。结论 :口腔CAFs的生长增殖特性发生了变化 ,推测可能是对肿瘤
Objective:To study the proliferation characteristics of the oral carcinoma-associated fibroblasts (CAFs).Method: The purified tongue CAFs and normal fibroblasts (NFs) of passage 3 that have been preserved before were resuscitated. Treated with the same culture condition, inoculation quantity and observing time, CAFs and NFs were compared of their proliferation characteristics, such as growth curve, double times of colony and mitotic index curve MTT method was used to assay the vitality of the two kinds of cells. Result: In the general ,the growth speed,proliferation and mitosis ability and vitality of the CAFs and NFs (p<0.05).The double times of colony CAFs and NFs was 60.19 h and 76.61 hrespectively.Conclusion:The proliferation characteristics of CAFs have changed significantly ,which is supposed to be one of the mechanisms modulating the microecology of the oral tumor-host interface.
出处
《临床口腔医学杂志》
2004年第4期244-246,共3页
Journal of Clinical Stomatology
基金
教育部博士点基金资助 (2 0 0 30 61 0 0 51 )
教育部回国人员启动基金资助 (2 0 0 340 60 6)
关键词
成纤维细胞
癌
鳞状细胞
口腔
增殖
fibroblasts
carcinoma
squamous cell
oral
proliferation
