摘要
用126_Ⅰ标记的纤维蛋白测定淫羊藿总黄酮甙(TFG)对小鼠巨噬细胞(M)纤溶活性的影响,用专一发色底物法测定在TFG作用下小鼠M的纤溶酶原激活剂(PA)的活性。结果表明TFG能使小鼠M中PA的活性显著增加,酶活性相当于0.731 IU/ml(P<0.01)。鼠M纤溶活性也显著增强,给药组的纤溶活性是对照组的2.8倍(P<0.01)。SHRsp大鼠体内实验表明,TFG能显著降低SHRsp大鼠死亡率,并能抑制其血压的升高。对照组大鼠中风死亡率为90％,给药组死亡率5％。
The enhancement of fibrinolytic ability of stimulated murine macrophages by flavone glyeosides of Epimedium koreanum (TFG) was measured by the[125I]-fibrin-coated plate method. The activity of the plasminogen activator (PA) induced by TFG was determined by a spectrophotometric assay. The activity of PA produced by TFG-stimulated macrophages was 0.731 IU/ML. (P<0.01). TFG-stimulated macrophages showed rapid fibrinolysis. The activity of stimulated macrophages was approximately 2.8 fold that of the control. In in vivo experiments, the effect of TFG on spontaneously hypertensive and apoplexyrats (SHRsp) was very evident. The abiotic rate of TFG-stimulated rats was 5%, while that of the control group was 90%. TFG showed obvious hypotensi-ve activity as well.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1992年第6期419-423,共5页
Acta Academiae Medicinae Sinicae
关键词
淫羊藿
黄酮类
纤溶酶原
巨噬细胞
flavone glycosides of Epimedium Koreanum (TFG) plasminogenactivator macrophages spontaneously hypertensive and apoplexy rats (SHRsp)
