摘要
目的 检测多肿瘤抑制基因 1(multipletumorsuppressor 1,MTS1)基因在口腔黏膜癌前病变和鳞癌中的表达和变异 (纯合性缺失和突变 )情况 ,以期发现MTS1在口腔黏膜恶变发生发展中的作用。方法 采用免疫组化SP法检测MTS1的表达情况 ;同时采用PCR、SSCP技术检测相同标本中MTS1基因exon1和exon2的纯合性缺失和突变情况。结果 口腔癌前病变中MTS1基因全部表达 ,无基因缺失和突变。鳞癌中MTS1的表达阳性率 6 0 % ;有 10例发生exon1和 (或 )exon2的纯合性缺失 ,4例基因突变 ,总变异率为 31.1% (14 / 4 5 ) ;伴有局部淋巴结转移的鳞癌的基因变异率为5 7.1% ,不伴有淋巴结转移的为 8.3% ,两者间差异有显著性 (P <0 .0 5 )。结论 MTS1基因在口腔癌前病变中无改变 ,不能作为检测口腔癌前病变的生物学指标 ;MTS1基因改变在口腔鳞癌的发生。
Objective To investigate the expression and alteration(including homozygous deletion and mutation) of MTS1 gene in precancerous lesions and squamous cell carcinomas(SCC) of oral mucosa, and to analyse the function of MTS1 gene alteration in oral mucosal carcinogenesis. Methods The expression of p16 protein producted by MTS1 gene was examined with immunohistochemical SP method in 10 normal oral mucosas, 30 precancerous lesions(10 mild, 10 moderate and 10 severe dysplasia respectively )and 45 squamous cell carcinomas(SCCⅠ18, SCCⅡ 19, SCCⅢ 8). The deletion and mutation of exon1 and exon2 of MTS1 gene were examined with methods of PCR and SSCP in these same samples. Results All the precancerous lesions had p16 protein expression and no alteration of MTS1 gene. In SCC,the positive rate of p16 protein was 60.0% with 72.2% in SCCⅠ,57.9% in SCCⅡ,37.5% in SCC Ⅲ ,and there were no significant difference among the three groups by χ 2 test(P>0.05).Gene homozygous deletion of exon1 and/or exon2 was detected in 10 cases, and gene mutation in 4 cases. The whole rate of gene alteration was 31.1%(14/45).The MTS1 gene alteration rate was 27.8% in SCCⅠ, 31.6% in SCCⅡ, 37.5% in SCC Ⅲ and there was also no significant difference among the three groups by χ 2 test(P>0.05).In SCC with local lymph nodes metastasis,MTS1 alteration rate was 57.1%,while in SCC with no lymph nodes metastasis was 8.3%, and there was significant difference by χ 2 test(P<0.05). Conclusions MTS1 gene alteration is not an early event in the carcinogenesis of oral mucosa and can not be used as a biology mark to examine oral precancerous lesions. MTS1 gene may play a certain role in the progression of oral squamous cell carcinomas.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2003年第5期361-363,I004,共4页
Chinese Journal of Stomatology
基金
山东省自然科学基金资助项目 (Q97C0 412 8)
